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Titolo:
ROLE OF SP1 IN TRANSCRIPTIONAL ACTIVATION OF HUMAN NITRIC-OXIDE SYNTHASE TYPE-III GENE
Autore:
TANG JL; ZEMBOWICZ A; XU XM; WU KK;
Indirizzi:
UNIV TEXAS,HLTH SCI CTR,DEPT INTERNAL MED,DIV HEMATOL,POB 20708 HOUSTON TX 77225 UNIV TEXAS,HLTH SCI CTR,DEPT INTERNAL MED,DIV HEMATOL HOUSTON TX 77030
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 213, anno: 1995,
pagine: 673 - 680
SICI:
0006-291X(1995)213:2<673:ROSITA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOX-BINDING-PROTEINS; CELLS; EXPRESSION; PROMOTER; CLONING; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Tang et al., "ROLE OF SP1 IN TRANSCRIPTIONAL ACTIVATION OF HUMAN NITRIC-OXIDE SYNTHASE TYPE-III GENE", Biochemical and biophysical research communications, 213(2), 1995, pp. 673-680

Abstract

Endothelial nitric oxide synthase (eNOS or NOS-III) is constitutivelyexpressed. To elucidate the mechanism by which the basal expression of NOS-III gene is activated, we constructed in a luciferase vector, pXP1, serial 5'-deletion mutants of a 1.3-kb 5'-flanking fragment and transiently expressed them in cultured human endothelial cells. The promotor activity was detected in the -198/+22 region which contains several putative Sp1 binding sites. DNase I footprinting assays coupled with gel shift assays revealed the GC box (-104/-90) to be the Sp1 binding site. Site-directed mutation of 4 crucial bases in this site reducedthe promotor activity by > 90%. These findings provide strong evidence that binding of Sp1 or closely related protein to this site is required for the activation of basal NOS-III transcription. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:16:44