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Titolo:
REPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX I-A-BETA GENE-EXPRESSION BY DBPA AND DBPB (MYB-1) PROTEINS
Autore:
LLOBERAS J; MAKI RA; CELADA A;
Indirizzi:
UNIV BARCELONA,FAC BIOL,DEPT FISIOL,AV DIAGONAL 645 E-08028 BARCELONASPAIN UNIV BARCELONA,FAC BIOL,DEPT FISIOL IMMUNOL E-08028 BARCELONA SPAIN UNIV BARCELONA,FDN PI & SUNYER E-08907 LHOSPITALET LLOBR SPAIN LA JOLLA CANC RES FDN LA JOLLA CA 92037
Titolo Testata:
Molecular and cellular biology
fascicolo: 9, volume: 15, anno: 1995,
pagine: 5092 - 5099
SICI:
0270-7306(1995)15:9<5092:ROMHCI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MHC CLASS-II; DNA-BINDING PROTEINS; HERPES-SIMPLEX VIRUS; INTERFERON-GAMMA; NF-Y; MICE LACKING; B-CELL; TRANSCRIPTION; BOX; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
J. Lloberas et al., "REPRESSION OF MAJOR HISTOCOMPATIBILITY COMPLEX I-A-BETA GENE-EXPRESSION BY DBPA AND DBPB (MYB-1) PROTEINS", Molecular and cellular biology, 15(9), 1995, pp. 5092-5099

Abstract

The induction of major histocompatibility complex class II gene expression is mediated by three DNA elements in the promoters of these genes (W, X, and Y boxes), The U box contains an inverted CCAAT box sequence, and the binding activity to the CAAT box is mediated by factor NF-Y, which is composed of subunits NF-YA and NF-YB, We have found that transfection of either dbpA or dbpB (mYB-1) or both inhibits I-A beta gene expression, Although the genes for some members of the Y-box family of binding proteins have been isolated by screening an expression library using the Y-box sequence, under our conditions no binding of dbpA or dbpB to the Y box of the I-A beta or I-E alpha promoter was detected, This suggested that repression of I-A beta gene expression by dbpA and dbpB was not due to competition for binding to the Y-box sequence, The results suggest two other mechanisms by which dbpA and dbpB caninhibit transcription from the I-A beta promoter. When dbpA was added, the binding of NF-YA to DNA increased, which could be explained by interaction between these two proteins whose purpose is to increase thebinding affinity of NF-YA for DNA. However, this complex was unable to stimulate transcription from the I-A beta promoter, Thus, dbpA competed for the interaction between NF-YA and NF-YB by binding to NF-YA, When dbpB factor was added together with NF-YA and NB-YB, the binding of the NF-YA-NF-YB complex was reduced. This suggested that dbpB may compete with NF-YB for interaction with NF-YA, These results provide an example of how dbpA and dbpB may regulate transcription of promoters that utilize NF-Y as a transcription factor.

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Documento generato il 09/07/20 alle ore 23:22:07