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Titolo:
A DROSOPHILA SHC GENE-PRODUCT IS IMPLICATED IN SIGNALING BY THE DER RECEPTOR TYROSINE KINASE
Autore:
LAI KMV; OLIVIER JP; GISH GD; HENKEMEYER M; MCGLADE J; PAWSON T;
Indirizzi:
MT SINAI HOSP,SAMUEL LUNENFELD RES INST,PROGRAMME MOLEC BIOL & CANC,600 UNIV AVE TORONTO ON M5G 1X5 CANADA MT SINAI HOSP,SAMUEL LUNENFELD RES INST,PROGRAMME MOLEC BIOL & CANC TORONTO ON M5G 1X5 CANADA UNIV TORONTO,DEPT MOLEC & MED GENET TORONTO ON M5S 1A8 CANADA
Titolo Testata:
Molecular and cellular biology
fascicolo: 9, volume: 15, anno: 1995,
pagine: 4810 - 4818
SICI:
0270-7306(1995)15:9<4810:ADSGII>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUANINE-NUCLEOTIDE EXCHANGE; EGF RECEPTOR; SEVENLESS; PROTEIN; GRB2; RAS; PHOSPHORYLATION; ACTIVATION; ASSOCIATION; TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
K.M.V. Lai et al., "A DROSOPHILA SHC GENE-PRODUCT IS IMPLICATED IN SIGNALING BY THE DER RECEPTOR TYROSINE KINASE", Molecular and cellular biology, 15(9), 1995, pp. 4810-4818

Abstract

Antibodies to the human Shc adaptor protein were used to isolate a cDNA encoding a Drosophila Shc protein (dShc) by screening an expressionlibrary. The dshc gene, which maps to position 67B-C on the third chromosome, encodes a 45-kDa protein that is widely expressed throughout the Drosophila life cycle. In flies, the dShc protein physically associates with the activated Drosophila epidermal growth factor receptor homolog (DER) and is inducibly phosphorylated on tyrosine by DER. The 45-kDa dShc protein is closely related both in overall organization andin amino acid sequence (46% identity) to the 52-kDa mammalian Shc isoform. In addition to a C-terminal Src homology 2 (SH2) domain, dShc contains an N-terminal phosphotyrosine binding (PTB) domain, which associates in vitro with the autophosphorylated DER receptor tyrosine kinase and with phosphopeptides containing an Asn-Pro-X-pTyr motif, where pTyr stands for phosphotyrosine. A potential binding site for the dShc PTB domain is located at Tyr-1228 of DER. These results indicate that the shc gene has been conserved in evolution, as have the binding properties of the Shc PTB and SH2 domains. Despite the close relationship between the Drosophila and mammalian Shc proteins, dShc lacks the high-affinity Grb2-binding site found in mammalian Shc, suggesting that Shc proteins may have functions in addition to regulation of the Ras pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 12:41:51