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Titolo:
PROLIFERATION AND MIGRATION OF ENDOTHELIAL-CELLS IS PROMOTED BY ENDOTHELINS VIA ACTIVATION OF ET(B) RECEPTORS
Autore:
MORBIDELLI L; ORLANDO C; MAGGI CA; LEDDA F; ZICHE M;
Indirizzi:
UNIV FLORENCE,DEPT PRECLIN & CLIN PHARMACOL,VIALE MORGAGNI 65 I-50134FLORENCE ITALY UNIV FLORENCE,DEPT PRECLIN & CLIN PHARMACOL I-50134 FLORENCE ITALY MENARINI PHARMACEUT,DEPT PHARMACOL A I-50131 FLORENCE ITALY MARIO AIAZZI MANCINI,CLIN BIOCHEM UNIT I-50134 FLORENCE ITALY UNIV FLORENCE,DEPT CLIN PHYSIOPATHOL I-50134 FLORENCE ITALY
Titolo Testata:
American journal of physiology. Heart and circulatory physiology
fascicolo: 2, volume: 38, anno: 1995,
pagine: 686 - 695
SICI:
0363-6135(1995)38:2<686:PAMOEI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPEPTIDASE 24.11 ENKEPHALINASE; HUMAN UMBILICAL VEIN; NEUTRAL ENDOPEPTIDASE; PRIMARY CULTURE; DNA-SYNTHESIS; HYDROLYSIS; CLONING; EXPRESSION; SUBTYPES; INVIVO;
Keywords:
ENDOTHELIN RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
L. Morbidelli et al., "PROLIFERATION AND MIGRATION OF ENDOTHELIAL-CELLS IS PROMOTED BY ENDOTHELINS VIA ACTIVATION OF ET(B) RECEPTORS", American journal of physiology. Heart and circulatory physiology, 38(2), 1995, pp. 686-695

Abstract

The growth and migration of endothelial cells are the prerequisites for vascular remodeling. The effects of endothelin (ET)-1 and -3 (ET-1,ET-3) on the proliferation and migration of endothelial cells isolated from bovine adrenal capillaries (BACE) and human umbilical veins (HUVEC) have been investigated. Cell proliferation (measured as DNA synthesis and as total cell number) and migration were significantly increased by ET-1 and ET-3. Dose-dependent proliferation was produced by ET-1 and ET-3 in both cell lines, with maximal effects at 0.1 nM concentration. ET-1 and ET-3 also stimulated BACE and HUVEC mobilization in a dose-dependent manner. The maximal responses were obtained at 10 nM concentration in both BACE and HUVEC. The full agonist for the ET(B) receptor, ET-(16-21), was able to reproduce the effects of ET on proliferation and migration of both cell lines. Modification of ET-(16-21) at Leu(17) and Ile(19) and amidation of the COOH-terminal were accompanied by loss of activity. The ET(B)-receptor antagonist IRL-1038 blocked the migration induced by ET-3 and ET-(16-21), whereas the ETA-receptorantagonist BQ-123 was not effective. We conclude that ETs, by favoring endothelial cell growth and mobilization, can contribute to neovascularization through an autocrine mechanism that requires ET(B)-receptoractivation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 05:44:22