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Titolo:
GLOMERULAR MESSENGER-RNA EXPRESSION OF ANGIOTENSINASE-A AFTER RENAL ABLATION
Autore:
WOLF G; THAISS F; MUELLER E; DISSER M; POOTH R; ZAHNER G; STAHL RAK;
Indirizzi:
UNIV HAMBURG,HOSP EPPENDORF,DEPT MED,DIV NEPHROL & OSTEOL,PAVILION 61,MARTINISTR 52 D-20246 HAMBURG GERMANY
Titolo Testata:
Experimental nephrology
fascicolo: 4, volume: 3, anno: 1995,
pagine: 240 - 248
SICI:
1018-7782(1995)3:4<240:GMEOAA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Keywords:
ANGIOTENSIN II; RENIN; ENZYME ACTIVITY; RAMIPRIL; GLOMERULAR HEMODYNAMICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
G. Wolf et al., "GLOMERULAR MESSENGER-RNA EXPRESSION OF ANGIOTENSINASE-A AFTER RENAL ABLATION", Experimental nephrology, 3(4), 1995, pp. 240-248

Abstract

The local concentration of angiotensin II (ANG II) in the renal microenvironment is not only controlled by the generation of this peptide but also by its enzymatic degradation, Angiotensinase A (ATA; aminopeptidase A, A.C.3.4.11.7) is a major exopeptidase of the glomerulus involvement in the metabolism of ANG II. We studied the glomerular mRNA levels of ATA in a remnant kidney model 1 - 12 weeks after 1 1/3 nephrectomy. Functional parameters (systolic blood pressure and albuminuria) demonstrated the progression of renal disease in this model. GlomerularATA enzyme activity significantly increased 1-5 weeks after nephrectomy and returned to control levels 12 weeks after ablation, In general,changes in steady-state mRNA expression for ATA were rather small. mRNA expression for ATA in isolated glomeruli as evaluated by northern blots was slightly increased 1 and 3 weeks after 1 1/3 nephrectomy but was suppressed 5 and 12 weeks after renal ablation compared to age-matched 2-kidney controls. Treatment of animals with the ACE inhibitor ramipril for 5 and 12 weeks partly inhibited the decrease in ATA transcripts after 1 1/3 nephrectomy and stimulated expression in 2-kidney controls whereas the ACE inhibitor decreased glomerular ATA enzyme activity in nephrectomized rats at 5 weeks, Isolated glomeruli from normal controls superfused with 10(-6) M ANG II for 60 min demonstated no change in ATA transcripts. Our results show that ATA steady-state mRNA levels are slightly elevated early (1-3 weeks) after renal ablation, and are subsequently suppressed (5-12 weeks). ATA enzyme activity is also increased early and returned (12 weeks) to levels measured in age-matched 2-kidney controls, Reverse expression of ATA mRNA and enzyme activity in rats treated with ramipril may suggest a negative feedback regulation between enzyme activity and transcripts, Although these data suggest that ATA was dynamically regulated after renal ablation :and maybe involved in the modulation of local ANG II levels in the remnant kidney, it remains to be determined whether the observed relatively small changes in ATA mRNA expression are important in the adaptative regulation of this enzyme.

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Documento generato il 13/08/20 alle ore 14:15:05