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Titolo:
TIME-COURSE OF COPROANTIGEN EXCRETION IN ECHINOCOCCUS-MULTILOCULARIS INFECTIONS IN FOXES AND AN ALTERNATIVE DEFINITIVE HOST, GOLDEN-HAMSTERS
Autore:
NONAKA N; IIDA M; YAGI K; ITO T; OOI HK; OKU Y; KAMIYA M;
Indirizzi:
HOKKAIDO UNIV,GRAD SCH VET MED,PARASITOL LAB SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,GRAD SCH VET MED,PARASITOL LAB SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO INST PUBL HLTH SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
International journal for parasitology
fascicolo: 11, volume: 26, anno: 1996,
pagine: 1271 - 1278
SICI:
0020-7519(1996)26:11<1271:TOCEIE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYMENOLEPIS-DIMINUTA; INTESTINAL MUCUS; IMMUNODIAGNOSIS; TAENIASIS; RATS; DOGS;
Keywords:
ECHINOCOCCUS MULTILOCULARIS; COPROANTIGEN; HAMSTER; FOX; ALTERNATIVE DEFINITIVE HOST; DIAGNOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
N. Nonaka et al., "TIME-COURSE OF COPROANTIGEN EXCRETION IN ECHINOCOCCUS-MULTILOCULARIS INFECTIONS IN FOXES AND AN ALTERNATIVE DEFINITIVE HOST, GOLDEN-HAMSTERS", International journal for parasitology, 26(11), 1996, pp. 1271-1278

Abstract

Coproantigen excretion during experimental infections of Echinococcusmultilocularis in foxes and an alternative definitive host, golden hamsters, was evaluated by a sandwich ELISA using a monoclonal antibody. A sigmoidal increase of antigen excretion from the developing parasites was observed in in vitro incubation of the parasites collected on different days during the first 21 days post-infection (DPI). In hamsters, the ELISA O.D. value of faeces became positive at 4 DPI. Thereafter, the O.D. value increased in semi-sigmoidal fashion in the first 42 DPI, probably reflecting the development of the parasites. In foxes, the O.D. value became positive at 6 DPI. However, contrary to that in hamsters, after the initial steep rise, the O.D. value suddenly decreased to 1/2 the level during 15-17 DPI, indicating that a large number of worms might have been expelled. The parasite eggs were detected by the sugar centrifugal-flotation technique (Ito, Yagi & Ishige, 1989) from 29 to 84 DPI but not thereafter to 125 DPI, although mature parasites were detected at 125 DPI. In contrast, positive O.D. values were obtained almost constantly until 125 DPI, indicating that the coproantigen detection assay was more sensitive than the egg detection assay. The detection limit of the coproantigen detection assay was roughly estimated to be around 100 worms. These observations, along with the fact that the assay was designed to detect a heat-resistant coproantigen inheat-sterilized fecal samples, indicate that the coproantigen detection assay is a safe and useful method, not only for diagnosis in the definitive host of E. multilocularis, but also for monitoring parasite development and change in parasite burden during an experimental infection. Copyright (C) 1996 Australian Society for Parasitology. Publishedby Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:29:21