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Titolo:
SECONDARY STRUCTURE DETERMINATION BY NMR-SPECTROSCOPY OF AN IMMUNOGLOBULIN-LIKE DOMAIN FROM THE GIANT MUSCLE PROTEIN TITIN
Autore:
PFUHL M; GAUTEL M; POLITOU AS; JOSEPH C; PASTORE A;
Indirizzi:
EUROPEAN MOLEC BIOL LAB,MEYERHOFSTR 1 D-69117 HEIDELBERG GERMANY
Titolo Testata:
Journal of biomolecular NMR
fascicolo: 1, volume: 6, anno: 1995,
pagine: 48 - 58
SICI:
0925-2738(1995)6:1<48:SSDBNO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOELECTRON MICROSCOPY; CHEMICAL-SHIFT; GLOBULAR HEAD; M-BAND; SUPPRESSION; EXTENDS; TOCSY; WATER; CDNA;
Keywords:
MUSCLES; TITIN IG-LIKE DOMAIN; SECONDARY STRUCTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M. Pfuhl et al., "SECONDARY STRUCTURE DETERMINATION BY NMR-SPECTROSCOPY OF AN IMMUNOGLOBULIN-LIKE DOMAIN FROM THE GIANT MUSCLE PROTEIN TITIN", Journal of biomolecular NMR, 6(1), 1995, pp. 48-58

Abstract

We present the complete N-15 and H-1 NMR assignment and the secondarystructure of an immunoglobulin-like domain from the giant muscle protein titin. The assignment was obtained using homonuclear and N-15 heteronuclear 2D and 3D experiments. The complementarity of 3D TOCSY-NOESYand 3D N-15 NOESY-HSQC experiments, using WATERGATE for water suppression allowed an efficient assignment of otherwise ambiguous cross peaks and was helpful in overcoming poor TOCSY transfer for some amino acids. The secondary structure is derived from specific NOEs between backbone alpha- and amide protons, secondary chemical shifts of alpha-protons and chemical exchange for the backbone amide protons. It consists of eight beta-strands, forming two beta-sheets with four strands each,similar to the classical beta-sandwich of the immunoglobulin superfamily, as previously predicted by sequence analysis. Two of the beta-strands are connected by type II beta-turns; the first beta-strand forms a beta-bulge. The whole topology is very similar to the only intracellular immunoglobulin-like domain for which a structure has been determined so far, i.e., telokin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:19:13