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Titolo:
DEMONSTRATION OF NITRIC-OXIDE SYNTHASE (NOS) IN MARMOSETS BY NADPH DIAPHORASE (NADPH-D) HISTOCHEMISTRY AND NOS IMMUNOREACTIVITY
Autore:
GROZDANOVIC Z; NAKOS G; CHRISTOVA T; NIKOLOVA Z; MAYER B; GOSSRAU R;
Indirizzi:
FREE UNIV BERLIN,DEPT ANAT,KONIGIN LUISE STR 15 D-14195 BERLIN GERMANY FREE UNIV BERLIN,DEPT ANAT D-14195 BERLIN GERMANY DEPT ANAT & HISTOL BU-1431 SOFIA BULGARIA KARL FRANZENS UNIV GRAZ,DEPT PHARMACOL & TOXICOL A-8010 GRAZ AUSTRIA
Titolo Testata:
Acta histochemica
fascicolo: 3, volume: 97, anno: 1995,
pagine: 321 - 331
SICI:
0065-1281(1995)97:3<321:DONS(I>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
DORSAL-ROOT GANGLIA; SPINAL-CORD; NEURONS; RAT; LOCALIZATION;
Keywords:
NITRIC OXIDE SYNTHASE; NADPH DIAPHORASE; MARMOSET; CALLITHRIX JACCHUS; IMMUNOHISTOCHEMISTRY; CATALYTIC HISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
Z. Grozdanovic et al., "DEMONSTRATION OF NITRIC-OXIDE SYNTHASE (NOS) IN MARMOSETS BY NADPH DIAPHORASE (NADPH-D) HISTOCHEMISTRY AND NOS IMMUNOREACTIVITY", Acta histochemica, 97(3), 1995, pp. 321-331

Abstract

Since species interdiversity often prevents the extrapolation of laboratory rodent data to man and similar problems may exist for nitric oxide synthase (NOS), NADPH-d activity and immunohistochemistry of NOS were investigated in the New World monkey Callithrix jacchus (marmoset), which has been shown to be close to the human situation in many respects. Using the NADPHd reaction with beta-NADPH and nitroblue tetrazolium (NET) on acetone-chloroform pretreated cryosections, NET formazan was found in many neural and non-neural (e.g. diverse epithelia, striated muscle fibers, vascular endothelium) cells in numerous tissues and. organs. Prefixation with formaldehyde lowered the number of NADPH-d active sites and the amount of formazan with the exception of neuronalNADPH- d as did incubation of fresh or acetone-chloroform-pretreated sections for NADPH-d in the presence of 0.5% formaldehyde. When 1% formaldehyde or 0.5 mM permanganate were used significant amounts of formazan appeared only in central and peripheral neurons, vasal endothelial cells, small intestinal enterocytes, plasma membrane region of striated muscle fibers as well as arteriolar cells in the kidney; except for enterocytes, these observations were confirmed by NOS-immunohistochemistry which revealed in addition reactive cells in the thymus and intestinal lamina propria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:40:44