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Titolo:
ROLE OF PILI AND THE PHASE-VARIABLE PILC PROTEIN IN NATURAL COMPETENCE FOR TRANSFORMATION OF NEISSERIA-GONORRHOEAE
Autore:
RUDEL T; FACIUS D; BARTEN R; SCHEUERPFLUG I; NONNENMACHER E; MEYER TF;
Indirizzi:
MAX PLANCK INST BIOL,INFEKT BIOL ABT,SPEMANNSTR 34 D-72076 TUBINGEN GERMANY MAX PLANCK INST BIOL,INFEKT BIOL ABT D-72076 TUBINGEN GERMANY MAX PLANCK INST INFEKT BIOL D-10117 BERLIN GERMANY
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 17, volume: 92, anno: 1995,
pagine: 7986 - 7990
SICI:
0027-8424(1995)92:17<7986:ROPATP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORIZONTAL GENETIC EXCHANGE; ANTIGENIC VARIATION; EPITHELIAL-CELLS; DNA-SEQUENCE; BACTERIA; ORGANIZATION; CONVERSION; SECRETION; SURFACE;
Keywords:
HORIZONTAL GENETIC EXCHANGE; TYPE 4 PILI; DNA UPTAKE; MEMBRANE TRANSPORT; RECOMBINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
T. Rudel et al., "ROLE OF PILI AND THE PHASE-VARIABLE PILC PROTEIN IN NATURAL COMPETENCE FOR TRANSFORMATION OF NEISSERIA-GONORRHOEAE", Proceedings of the National Academy of Sciences of the United Statesof America, 92(17), 1995, pp. 7986-7990

Abstract

The Gram-negative bacterial pathogen Neisseria gonorrhoeae is naturally competent for transformation with species-related DNA. We show herethat two phase-variable pilus-associated proteins, the major pilus subunit (pilin, or PilE) and PilC, a factor known to function in the assembly and adherence of gonococcal pill, are essential for transformation competence. The PilE and PilC proteins are necessary for the conversion of linearized plasmid DNA carrying the Neisseria-specific DNA uptake signal into a DNase-resistant form. The biogenesis of typical pilus fibers is neither essential nor sufficient for this process. DNA uptake deficiency of defined piliated pilC1,2 double mutants can be complemented by expression of a cloned pilC2 gene in trans. The PilC defectcan also be restored by the addition of purified PilC protein, or better, pili containing PilC protein, to the mutant gonococci. Our data suggest that the two phase-variable pit proteins act on the bacterial cell surface and cooperate in DNA recognition and/or outer membrane translocation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:08:41