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Titolo:
IN-VIVO MODIFICATION OF 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE AND SULFATE BY INFUSION OF SODIUM-SULFATE, CYSTEINE, AND METHIONINE
Autore:
KIM HJ; MADHU C; CHO JH; KLAASSEN CD;
Indirizzi:
UNIV KANSAS,MED CTR,CTR ENVIRONM & OCCUPAT HLTH,DEPT PHARMACOL TOXICOL & THERAPEUT KANSAS CITY KS 66160 UNIV KANSAS,MED CTR,CTR ENVIRONM & OCCUPAT HLTH,DEPT PHARMACOL TOXICOL & THERAPEUT KANSAS CITY KS 66160
Titolo Testata:
Drug metabolism and disposition
fascicolo: 8, volume: 23, anno: 1995,
pagine: 840 - 845
SICI:
0090-9556(1995)23:8<840:IMO35A>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSINE 3'-PHOSPHATE 5'-PHOSPHOSULFATE; INORGANIC SULFATE; RAT-LIVER; ACETAMINOPHEN; HARMOL; ACID; CONJUGATION; METABOLISM; TISSUES; TAURINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Kim et al., "IN-VIVO MODIFICATION OF 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE AND SULFATE BY INFUSION OF SODIUM-SULFATE, CYSTEINE, AND METHIONINE", Drug metabolism and disposition, 23(8), 1995, pp. 840-845

Abstract

The importance of tissue sulfate concentrations in regulating 3'-phosphoadenosine 5'-phosphosulfate (PARS) synthesis is not known, Therefore, this study was conducted to determine the influence of increased availability of inorganic sulfate on steady-state PARS concentrations invarious tissues. To increase tissue sulfate concentrations, 2-16 mmol/kg of sodium sulfate and sulfur-containing amino acids (cysteine or methionine) were infused intravenously for 2 hr into pentobarbital-anesthetized rats. Serial blood samples were taken during the infusion andanalyzed for sulfate concentrations, After 2 hr of infusion, liver, kidney, and brain were removed for quantification of tissue PARS and sulfate concentrations. Infusion of sodium sulfate, cysteine, and methionine increased serum and tissue sulfate concentrations in a dose-and time-dependent manner, Serum sulfate concentrations increased from 0.8 to 14 mM during the infusion of sodium sulfate, whereas infusions of cysteine and methionine increased serum sulfate concentrations to 4.8 and 2.0 mM, respectively, Tissue sulfate concentrations also increased during sulfate infusion. Liver sulfate concentration increased from 0.8 to 4.8 mM, kidney concentration increased from 0.6 to 31 mM, and brain concentration increased from 0.1 to 0.6 mM. Similar to the serum sulfate concentrations, sulfate infusion was the most effective in increasing tissue sulfate concentrations, cysteine was intermediate, and methionine the least effective, Although sulfate concentrations in liver, kidney, and brain increased 6-, 50-, and B-fold by infusing sulfate,respectively; tissue PARS levels were not altered markedly. Hepatic PARS concentrations increased significantly (30-35%) only when infused with the higher doses (8 or 16 mmol/kg/2 hr) of sodium sulfate, PAPS concentrations in kidney and brain were not influenced by the increase in tissue sulfate concentrations, In summary, infusions of sodium sulfate, cysteine, and methionine enhanced dramatically sulfate concentrations in serum, liver, kidney, and brain, but had minimal effect on tissue PARS concentrations. Therefore, it seems that steady-state PAPS concentrations in tissues are not readily modified by the enhancement oftissue sulfate concentrations.

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Documento generato il 12/07/20 alle ore 06:53:48