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Titolo:
SELECTIVE DISULFIDE LINKAGE OF PLANT THIONINS WITH OTHER PROTEINS
Autore:
PINEIRO M; DIAZ I; RODRIGUEZPALENZUELA P; TITARENKO E; GARCIAOLMEDO F;
Indirizzi:
UNIV POLITECN MADRID,ETS INGN AGRON,DEPT BIOTECNOL E-28040 MADRID SPAIN UNIV POLITECN MADRID,ETS INGN AGRON,DEPT BIOTECNOL E-28040 MADRID SPAIN
Titolo Testata:
FEBS letters
fascicolo: 2-3, volume: 369, anno: 1995,
pagine: 239 - 242
SICI:
0014-5793(1995)369:2-3<239:SDLOPT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Keywords:
THIONIN; TOXIN; NEOMYCIN PHOSPHOTRANSFERASE; BETA-GLUCURONIDASE; DISULFIDE BRIDGE; PSEUDOMONAS SOLANACEARUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
M. Pineiro et al., "SELECTIVE DISULFIDE LINKAGE OF PLANT THIONINS WITH OTHER PROTEINS", FEBS letters, 369(2-3), 1995, pp. 239-242

Abstract

Thionins are shown to form disulphide linkages with other proteins. The reaction with bacterial enzymes beta-glucuronidase and neomycin phosphotransferase II could be prevented and reversed with dithiothreitoland blocked with N-ethylmaleimide. Other cysteine-rich low-molecular-weight toxic peptides from plants (LTP-3 from barley and P19 from potato) did not react as the thionins. Certain cysteine-containing proteins, such bovine serum albumin, ovalbumin and cytochrome c, reacted withthionins, while others, including carbonic anhydrase, soybean trypsininhibitor, bovine-lung trypsin inhibitor and phosphorylase B did not. Selectivity of the reaction with a periplasmic component of the phytopathogenic bacterium Pseudomonas solanacearum was also shown.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 23:24:34