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Titolo:
PROGRAMMED CELL-DEATH IN SYMPATHETIC NEURONS - A STUDY BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS USING COMPUTER IMAGE-ANALYSIS
Autore:
AMESS B; TOLKOVSKY AM;
Indirizzi:
UNIV CAMBRIDGE,DEPT BIOCHEM,TENNIS COURT RD CAMBRIDGE CB2 1QW ENGLAND
Titolo Testata:
Electrophoresis
fascicolo: 7, volume: 16, anno: 1995,
pagine: 1255 - 1267
SICI:
0173-0835(1995)16:7<1255:PCISN->2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; IMMOBILIZED PH GRADIENTS; TISSUE TRANSGLUTAMINASE; PROTEIN-SYNTHESIS; 1ST DIMENSION; RNA-SYNTHESIS; CYCLIC-AMP; APOPTOSIS; DEPOLARIZATION; EXPRESSION;
Keywords:
APOPTOSIS; PROTEIN EXPRESSION; QUEST II; 2ND-DIMENSIONAL ELECTROPHORESIS SYSTEM; STORAGE PHOSPHOR IMAGING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
B. Amess e A.M. Tolkovsky, "PROGRAMMED CELL-DEATH IN SYMPATHETIC NEURONS - A STUDY BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS USING COMPUTER IMAGE-ANALYSIS", Electrophoresis, 16(7), 1995, pp. 1255-1267

Abstract

The technique of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) coupled with computer image analysis was used in this study to examine changes in protein expression occurring during the onset ofprogrammed cell death (PCD) in rat sympathetic neurons following withdrawal of nerve growth factor (NGF). Sympathetic neurons from superiorcervical ganglia of postnatal day-one Wistar rats were cultured in the presence of NGF for 24 h and then either maintained in the presence of NGF or deprived of NGF for a period of 8 h. To label the proteins being synthesised, neurons were cultured in the presence of L-[S-35]methionine for a further 2 h under the same conditions but with 3 % of the normal methionine concentration. Neuronal proteins were then analysed by 2-D PAGE using immobilised pH gradient (IPG) gel strips id the first dimension. For the second dimension a custom-built electrophoresissystem capable of running multiple sodium dodecyl sulfate (SDS)-PAGE slab gels in a vertical configuration, with good temperature control (/- 0.7 degrees C) was used and is described in this paper. Proteins resolved on the dried gels were visualised using storage phosphor technology and the digitised images subjected to rigorous analysis using the QUEST II software system. Seventeen proteins whose relative synthesis decreased and four proteins that increased upon NGF withdrawal were located and are documented.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/08/20 alle ore 05:13:08