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Titolo:
CYTOKINE-CONTAINING LIPOSOMES AS ADJUVANTS FOR HIV SUBUNIT VACCINES
Autore:
LACHMAN LB; SHIH LCN; RAO XM; HU XS; BUCANA CD; ULLRICH SE; CLELAND JL;
Indirizzi:
UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CELL BIOL,1515 HOLCOMBE BLVD HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT IMMUNOL HOUSTON TX 77030 GENENTECH INC S SAN FRANCISCO CA 94080
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 8, volume: 11, anno: 1995,
pagine: 921 - 932
SICI:
0889-2229(1995)11:8<921:CLAAFH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED IMMUNE-RESPONSES; IMMUNOLOGICAL ADJUVANTS; INTERLEUKIN-12; ANTIGENS; INVIVO; IL-1; MOLECULES; INDUCTION; BIOLOGY; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
L.B. Lachman et al., "CYTOKINE-CONTAINING LIPOSOMES AS ADJUVANTS FOR HIV SUBUNIT VACCINES", AIDS research and human retroviruses, 11(8), 1995, pp. 921-932

Abstract

Dehydration-rehydration liposome vesicles (DRVs) containing various cytokines were evaluated for their ability to induce delayed-type hypersensitivity (DTH) and humoral immunity to the recombinant envelope protein rgp120 of the MN strain of human immunodeficiency virus type 1 (HIV-1). The DRVs trapped similar to 25% of the radiolabeled cytokines and similar to 17% of the radiolabeled rgp120 that were added. The level of trapping was greater than the aqueous volume of the DRVs, indicating association of the proteins with the lipid bilayer, Flow cytometric analysis using antibody to rgp120 or the V3 loop of rgp120 showed the diameter of the DRVs to be 2-7.5 mu m. Transmission electron microscopy confirmed the heterogeneity in size of the DRVs and revealed morphological heterogeneity, Transmission electron microscopy with immunogold labeling also revealed the presence of rgp120 on the surface of theDRVs. In vitro bioassays demonstrated slow leakage of biologically active cytokines from DRVs soaked in tissue culture medium containing serum. Mice injected subcutaneously three times at 14-day intervals withDRVs containing 15 mu g of rgp120 plus interleukin 6 (IL-6) or interferon gamma (IFN-gamma) produced significantly greater DTH responses than mice injected with DRVs containing rgp120 alone. Soluble rgp120 plus soluble IFN-gamma produced DTH in some experiments, but of lower magnitude than the comparable DRVs. Interleukin 6, but not IFN-gamma, increased the antibody titer to rgp120 when included in the DRVs. The mice did not develop antibodies to IFN-gamma or IL-6. Induction of DTH byvaccines may increase protection from viral pathogens such as HIV. Cytokine-containing liposomes may be an effective adjuvant for the induction of a DTH response to envelope-antigen subunit vaccines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 19:55:03