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Titolo:
N-15, C-13, AND H-1-NMR ASSIGNMENTS AND SECONDARY STRUCTURE FOR T4-LYSOZYME
Autore:
FISCHER MWF; MAJUMDAR A; DAHLQUIST FW; ZUIDERWEG ERP;
Indirizzi:
UNIV MICHIGAN,DIV BIOPHYS RES,930 N UNIV ANN ARBOR MI 48109 UNIV MICHIGAN,DIV BIOPHYS RES ANN ARBOR MI 48109 UNIV MICHIGAN,DEPT BIOL CHEM ANN ARBOR MI 48109 UNIV OREGON,INST MOLEC BIOL EUGENE OR 97403
Titolo Testata:
Journal of magnetic resonance. Series B
fascicolo: 2, volume: 108, anno: 1995,
pagine: 143 - 154
SICI:
1064-1866(1995)108:2<143:NCAHAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULSED-FIELD GRADIENTS; BACTERIOPHAGE-T4 LYSOZYME; LARGER PROTEINS; T4 LYSOZYME; NOESY-HMQC; SPECTROSCOPY; SPECTRA; MUTANT; RESOLUTION; RESONANCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M.W.F. Fischer et al., "N-15, C-13, AND H-1-NMR ASSIGNMENTS AND SECONDARY STRUCTURE FOR T4-LYSOZYME", Journal of magnetic resonance. Series B, 108(2), 1995, pp. 143-154

Abstract

Sequence-specific H-1, C-13, and N-15 backbone assignments and H-1 and C-13 side-chain assignments have been identified for T4-1ysozyme (164 residues, MW = 18.7 kDa). A variety of double- and triple-resonance 3D techniques were used, Some of these methods were applied in unconventional ways and a detailed description of the advantages and disadvantages of these approaches is given. Complete backbone resonances for 162 of the 164 residues and partial assignments for the remaining 2 residues were obtained. The H-1 and N-15 assignments are in agreement with those obtained previously by McIntosh et al, who used selective labeling (L. P. McIntosh et al., Biochemistuy 29, 6341 (1990)). Complete proton and carbon side-chain assignments were made for 120 residues andpartial side-chain assignments were made for 42 additional residues. A qualitative analysis of the medium-range NOESY data reveals a secondary structure consistent with the X-ray crystallographic structure. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 20:58:01