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Titolo:
CROSS-LINKING OF CD27 IN THE PRESENCE OF CD28 COSTIMULATION RESULTS IN T-CELL PROLIFERATION AND CYTOKINE PRODUCTION
Autore:
SUNDERPLASSMANN R; PICKL WF; MAJDIC O; KNAPP W; HOLTER W;
Indirizzi:
UNIV VIENNA,VIENNA INT RES COOPERAT CTR,INST IMMUNOL VIENNA AUSTRIA ST ANNA CHILDRENS HOSP,CHILDRENS CANC RES INST A-1090 VIENNA AUSTRIA UNIV VIENNA,INST IMMUNOL VIENNA AUSTRIA
Titolo Testata:
Cellular immunology
fascicolo: 1, volume: 164, anno: 1995,
pagine: 20 - 27
SICI:
0008-8749(1995)164:1<20:COCITP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; TUMOR-NECROSIS-FACTOR; HUMAN MONONUCLEAR-CELLS; RESTING LYMPHOCYTES-T; MESSENGER-RNA LEVELS; MOLECULAR-CLONING; SIGNAL TRANSDUCTION; CROSS-LINKING; INTERLEUKIN-4 PRODUCTION; ACTIVATION ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
R. Sunderplassmann et al., "CROSS-LINKING OF CD27 IN THE PRESENCE OF CD28 COSTIMULATION RESULTS IN T-CELL PROLIFERATION AND CYTOKINE PRODUCTION", Cellular immunology, 164(1), 1995, pp. 20-27

Abstract

Monoclonal antibodies recognizing CD27, a member of the nerve growth factor receptor family, have been observed to enhance or inhibit PHA- or CD3 mAb-stimulated T cell proliferation. In this study, we investigate the effects of CD27 stimulation on the proliferation and cytokine production of T cells stimulated simultaneously through CD3, CD2, or CD28. Here we report that simultaneous crosslinking of CD27 plus CD28 results in T cell proliferation and in the production of IL-2, IL-4, IL-10, and IFN-gamma. CD27 plus CD28 stimulation thus introduces a novelAg-independent pathway of T cell activation. We further show that allmajor T cell subsets (CD4(+), CD8(+), CD4(+)CD45RA(+), or CD4(+)CD45RO(+) T cells) respond to CD27 plus CD28-mediated costimulation. Synergistic effects on T cell stimulation are also found when CD27 is crosslinked on cells stimulated simultaneously through CD3 or CD2. In further experiments we show that crosslinking of CD27 elevates cytoplasmic free Ca2+ levels. Finally, both the CD3 plus CD27 and the CD28 plus CD27-stimulated T cell proliferation is sensitive to inhibition by CsA. Taken together, these data emphasize the importance of CD27 stimulationin the context of simultaneously received signals through CD3, CD2 or, most importantly, through CD28. Furthermore, signaling through CD27 appears to involve Ca2+-dependent mechanisms sensitive to CsA. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:22:24