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Titolo:
TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS
Autore:
POIRIER V; TYLER SJ; SLEEMAN J; DIFFIN F; BROWN KW;
Indirizzi:
SCH MED SCI,DEPT PATHOL & MICROBIOL,CLIC,RES UNIT,UNIV WALK BRISTOL BS8 1TD AVON ENGLAND
Titolo Testata:
International journal of oncology
fascicolo: 3, volume: 7, anno: 1995,
pagine: 623 - 630
SICI:
1019-6439(1995)7:3<623:TAWC1A>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH FACTOR-II; WILMS-TUMOR; EPITHELIAL-CELLS; INSULIN GENE; HETEROZYGOSITY; REGION; LINE; EXPRESSION; DNA; WT1;
Keywords:
11P15 LOCUS; HUMAN KIDNEY CELLS; SV40; WILMS TUMOR GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
V. Poirier et al., "TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS", International journal of oncology, 7(3), 1995, pp. 623-630

Abstract

Chromosome 11p15 has been suggested to be a potential site for a second Wilms' tumour gene (a childhood nephroblastoma). Human foetal kidney cells and normal kidney cells from Wilms' tumour patients were transformed with SV40 derivative vectors. As some of the cell lines progressed to tumorigenicity, we observed that chromosome 11p13, site of the WT1 suppressor gene, did not show any allelic loss. However, RFLP analysis showed that chromosome 11p15 was affected by allelic losses on different genes in some cell lines but not necessarily prior to the appearance of tumorigenicity. We also observed that the most aggressive cell Line (SVCU/NK), derived from the normal kidney cells of a Wilms' tumour patient, showed increased expression of c-Ha-ras proto-oncogene at later passage and in the tumour tissue extracted from nude mice. Finally we report a lack of tumour suppression activity of one cell line SVT1B6/NK, when fused with the tumorigenic G401 cell line (the latter has been used in tumour suppression experiments as a Wilms' tumour cell line before being identified as a Rhabdoid tumour cell line). These experiments are consistent with the existence of a suppressor gene at chromosome 11p15.

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Documento generato il 28/11/20 alle ore 21:27:33