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Titolo:
CRYOPRESERVATION OF IN VITRO-GROWN APICAL MERISTEMS OF WASABI (WASABIA-JAPONICA) BY ENCAPSULATION-VITRIFICATION METHOD
Autore:
MATSUMOTO T; SAKAI A; TAKAHASHI C; YAMADA K;
Indirizzi:
SHIMANE AGR EXPTL STN,2440 ASHIWATA IZUMO SHIMANE 693 JAPAN YOKOTA TOWN OFF NITA SHIMANE 69918 JAPAN
Titolo Testata:
Cryo-letters
fascicolo: 4, volume: 16, anno: 1995,
pagine: 189 - 196
SICI:
0143-2044(1995)16:4<189:COIVAM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSEQUENT PLANT-REGENERATION; VAR BRASILIENSIS TANAKA; SIMPLE FREEZING METHOD; CITRUS-SINENSIS OSB; SHOOT-TIPS; NUCELLAR CELLS; INVITRO; DEHYDRATION; PEAR; MULBERRY;
Keywords:
ALGINATE COATED BEADS; CRYOPRESERVATION; ENCAPSULATION-VITRIFICATION METHOD; WASABI; VITRIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
T. Matsumoto et al., "CRYOPRESERVATION OF IN VITRO-GROWN APICAL MERISTEMS OF WASABI (WASABIA-JAPONICA) BY ENCAPSULATION-VITRIFICATION METHOD", Cryo-letters, 16(4), 1995, pp. 189-196

Abstract

Alginate-coatcd apical meristems from in vitro-grown wasabi (Wasabia japonica Matsumura) were successfully cryopreserved following dehydration by vitrification solutions. Excised meristems precultured oil solidified 1/2 MS medium containing 0.3 M sucrose at 20 degrees C for 1 day were trapped into alginate-coated beads containing a mixture of 2 M glycerol plus 0.4 M sucrose. These encapsulated meristems were dehydrated with a highly concentrated vitrification solution (designated PVS2or PVS3) for about 30 min at 25 degrees C or 70 to 100 min at 0 degrees C prior to a plunge into liquid nitrogen. Successfully encapsulatedvitrified meristems resumed growth within 3 days and the average rateof shoot formation amounted to about 95%. The rate of shoot formationof encapsulated vitrified meristems was higher by as much as 30% thanthe meristems cryopreserved by the encapsulation-dehydration technique. Besides, the recovery growth was much earlier than the latter. The encapsulation-vitrification method is easy to handle and saves greatlythe time used for dehydration. Thus, the encapsulation-vitrification method promises for cryopreserving meristems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 12:24:22