Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PREVALENCE OF DIENTAMOEBA-FRAGILIS ANTIBODIES IN CHILDREN AND RECOGNITION OF A 39 KDA IMMUNODOMINANT PROTEIN ANTIGEN OF THE ORGANISM
Autore:
CHAN F; STEWART N; GUAN M; ROBB I; FUITE L; CHAN I; DIAZMITOMA F; KING J; MACDONALD N; MACKENZIE A;
Indirizzi:
CHILDRENS HOSP EASTERN ONTARIO,DEPT LAB MED OTTAWA ON K1H 8L1 CANADA CHILDRENS HOSP EASTERN ONTARIO,DEPT PEDIAT OTTAWA ON K1H 8L1 CANADA OTTAWA CIVIC HOSP,DIV MICROBIOL OTTAWA ON K1Y 4E9 CANADA TEMPLE UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL PHILADELPHIA PA 19140
Titolo Testata:
European journal of clinical microbiology & infectious diseases
fascicolo: 12, volume: 15, anno: 1996,
pagine: 950 - 954
SICI:
0934-9723(1996)15:12<950:PODAIC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL PARASITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
F. Chan et al., "PREVALENCE OF DIENTAMOEBA-FRAGILIS ANTIBODIES IN CHILDREN AND RECOGNITION OF A 39 KDA IMMUNODOMINANT PROTEIN ANTIGEN OF THE ORGANISM", European journal of clinical microbiology & infectious diseases, 15(12), 1996, pp. 950-954

Abstract

Dientamoeba fragilis, a common intestinal protozoan parasite in Canada, has been associated with diarrhoea and abdominal pain in some patients. Seroprevalence of this organism has not been reported previously. In the present study sera from three symptomatic patients, 12 age- and sex-matched controls, and 189 randomly selected healthy individuals (age 6 months to 19 years) were tested for antibodies against Dientamoeba fragilis by an indirect immunofluorescence (IIF) assay. All three symptomatic patients infected with Dientamoeba fragilis had positive IIF titres of 80, and all 12 matched controls had positive titres ranging 20 to 160 (geometric mean titre 48). Of the 189 healthy children, 172 (91%) were positive at a serum dilution of 1:10 or higher. The specificity of the IIF assay was reinforced by immunoblotting 20 representative serum samples against Dientamoeba fragilis. In all 17 IIf-positive serum samples, a 39 kDa protein band of Dientamoeba fragilis was identified, the same band recognized by a mouse monoclonal antibody raised in our laboratory. Findings over a five-year period indicate that Dientamoeba fra-g-ilis was the most common protozoan, followed closely by Giardia lamblia and more distantly by Cryptosporidium parvum. The high seropositivity of 91% for Dientamoeba fragilis compares reasonablywell with serologic data obtained by IIF and reported previously for Giardia lamblia (85.6%) and Cryptosporidium parvum (86%).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:29:28