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Titolo:
EXPRESSION OF GABA RECEPTOR RHO-1 AND RHO-2 SUBUNITS IN THE RETINA AND BRAIN OF THE RAT
Autore:
ENZ R; BRANDSTATTER JH; HARTVEIT E; WASSLE H; BORMANN J;
Indirizzi:
MAX PLANCK INST HIRNFORSCH,DEUTSCHORDENSTR 46 D-60528 FRANKFURT GERMANY MAX PLANCK INST HIRNFORSCH D-60528 FRANKFURT GERMANY
Titolo Testata:
European journal of neuroscience
fascicolo: 7, volume: 7, anno: 1995,
pagine: 1495 - 1501
SICI:
0953-816X(1995)7:7<1495:EOGRRA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; ROD BIPOLAR CELLS; PROTEIN KINASE-C; XENOPUS OOCYTES; CEREBELLAR MEMBRANES; PHARMACOLOGY; CHANNELS; NEURONS; BINDING; INVITRO;
Keywords:
GABA(C) RECEPTOR; BIPOLAR CELLS; RT-PCR; IN SITU HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
R. Enz et al., "EXPRESSION OF GABA RECEPTOR RHO-1 AND RHO-2 SUBUNITS IN THE RETINA AND BRAIN OF THE RAT", European journal of neuroscience, 7(7), 1995, pp. 1495-1501

Abstract

We have investigated the distribution of GABA receptor rho 1 and rho 2 subunits in the rat central nervous system. Cloning of rat rho 1 andrho 2 cDNA fragments revealed similarities to the corresponding humansequences of 99% (rho 1) and 88% (rho 2) at the protein level. Whereas the human rho 2 subunit has no consensus sequence for phosphorylation by protein kinase C, the cytoplasmic loop of the rat sequence contains two such sites. Use of the polymerase chain reaction with reverse-transcribed total RNA (RT-PCR) from different brain tissues revealed that transcript for the rho 1 subunit was present in the retina only. The rho 2 mRNA was detected in all brain regions, with the highest levelof expression in the retina. In situ hybridization of retinal sections revealed that rho 1 and rho 2 transcripts are present in the inner nuclear layer. RT-PCR and in situ hybridization of isolated retinal cells showed that both rho subunits are present in rod bipolar cells. Since these cells express bicuculline-insensitive GABA receptors, our results further support the idea that rho subunits are part of the GABA(C) receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 19:11:29