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Titolo:
EXPRESSION OF THE MESSENGER-RNA OF 7 METABOTROPIC GLUTAMATE RECEPTORS(MGLUR1 TO MGLUR7) IN THE RAT RETINA - AN IN-SITU HYBRIDIZATION STUDYON TISSUE-SECTIONS AND ISOLATED CELLS
Autore:
HARTVEIT E; BRANDSTATTER JH; ENZ R; WASSLE H;
Indirizzi:
MAX PLANCK INST HIRNFORSCH,NEUROANAT ABT,DEUTSCHORDENSTR 46 D-60528 FRANKFURT GERMANY MAX PLANCK INST HIRNFORSCH,NEUROANAT ABT D-60528 FRANKFURT GERMANY
Titolo Testata:
European journal of neuroscience
fascicolo: 7, volume: 7, anno: 1995,
pagine: 1472 - 1483
SICI:
0953-816X(1995)7:7<1472:EOTMO7>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY AMINO-ACIDS; CENTRAL-NERVOUS-SYSTEM; ROD BIPOLAR CELLS; INOSITOL PHOSPHOLIPID-METABOLISM; HORIZONTAL CELLS; SIGNAL TRANSDUCTION; HIPPOCAMPAL-NEURONS; MAMMALIAN RETINA; MESSENGER-RNA; 2-AMINO-4-PHOSPHONOBUTYRIC ACID;
Keywords:
GLUTAMATE; METABOTROPIC RECEPTORS; IN SITU HYBRIDIZATION; OLIGONUCLEOTIDE PROBES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
E. Hartveit et al., "EXPRESSION OF THE MESSENGER-RNA OF 7 METABOTROPIC GLUTAMATE RECEPTORS(MGLUR1 TO MGLUR7) IN THE RAT RETINA - AN IN-SITU HYBRIDIZATION STUDYON TISSUE-SECTIONS AND ISOLATED CELLS", European journal of neuroscience, 7(7), 1995, pp. 1472-1483

Abstract

We have studied the expression of mRNAs for seven metabotropic glutamate receptors (mGluR1-7) in the retina of the adult rat by in situ hybridization with tissue sections and isolated cells using [alpha(35)S]dATP-labelled oligonucleotide probes. Hybridization revealed the expression of six of the metabotropic receptor mRNAs, mGluR1, 2 and 4-7, in the retina, white mGluR3 was not detected. Each of the expressed receptor mRNAs showed a distinct pattern of expression. In the outer nuclear layer, corresponding to photoreceptor somata, no labelling was detected. In the outer part of the inner nuclear layer, putative horizontalcells were labelled for mGluR5. More proximal in this layer, corresponding to the position of bipolar cell somata, there was strong labelling for mGluR6. A small number of bipolar cells were also labelled for mGluR5 and mGluR7. In situ hybridization with isolated cells showed that mGluR6 was expressed by rod bipolar cells. Subsets of amacrine cells, with cell bodies along the border between the inner nuclear layer and the inner plexiform layer, were positive for mGluR1, 2, 4 and 7, suggesting considerable heterogeneity of these receptors among amacrine cells. None of the seven metabotropic receptor mRNAs was expressed in isolated Muller glial cells. In the ganglion cell layer, virtually every ganglion cell and displaced amacrine cell was labelled for mGluR1 and mGluR4. Some cells in this layer (-20% of the total), most likely both ganglion cells and displaced amacrine cells, were also labelled for mGluR2 and mGluR7. These findings suggest that metabotropic glutamate receptors are considerably more widespread among neurons in the retina than indicated by previous physiological and pharmacological investigations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:11:47