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Titolo:
KARYOTYPE STUDIES ON DIFFERENT STRAINS OF CANDIDA-MOLISCHIANA BY PULSED-FIELD GEL-ELECTROPHORESIS
Autore:
JANBON G; MAGNET R; BIGEY F; ARNAUD A; GALZY P;
Indirizzi:
ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES,PL PIERRE VIALA F-34060 MONTPELLIER 1 FRANCE ECOLE NATL SUPER AGRON MONTIPELLIER,CHAIR MICROBIOL IND & GENET MICOORGANISMES F-34060 MONTPELLIER 1 FRANCE
Titolo Testata:
Current genetics
fascicolo: 2, volume: 28, anno: 1995,
pagine: 150 - 154
SICI:
0172-8083(1995)28:2<150:KSODSO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
KLUYVEROMYCES-MARXIANUS; DNA-MOLECULES; ALBICANS; YEASTS; CHROMOSOMES; ASSIGNMENT; IDENTIFICATION; SACCHAROMYCES; SEPARATION; GLABRATA;
Keywords:
ELECTROPHORETIC KARYOTYPE; CANDIDA MOLISCHIANA; DE-REPRESSED MUTANT; BETA-GLUCOSIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
G. Janbon et al., "KARYOTYPE STUDIES ON DIFFERENT STRAINS OF CANDIDA-MOLISCHIANA BY PULSED-FIELD GEL-ELECTROPHORESIS", Current genetics, 28(2), 1995, pp. 150-154

Abstract

We used pulsed-field gel electrophoresis to compare the electrophoretic karyotype of a Candida molischiana mutant, de-repressed for beta-glucosidase production, with a wild-type strain and a reference strain. The chromosomal organization in this mutant yeast was found to be quite different, Hybridization patterns and the relative fluorescence of all bands indicated eight chromosomes in the mutant strain and seven inthe other two, All three strains seemed to be haploid, with an estimated genome size of 12 Mb; the beta-glucosidase gene was on the same chromosome in all of them and the SfiI restriction patterns of this chromosome indicated that it is not affected by the mutation.

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Documento generato il 04/04/20 alle ore 19:04:39