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Titolo:
MEASURING RESISTANCE TO PHAGOCYTOSIS OF GROUP-A AND GROUP-G STREPTOCOCCI - COMPARISON OF DIRECT BACTERICIDAL ASSAY AND FLOW-CYTOMETRY
Autore:
SCHNITZLER N; HAASE G; BUSSING A; KAUFHOLD A; BEYHS P; PODBIELSKI A;
Indirizzi:
RHEIN WESTFAL TH AACHEN,INST MED MICROBIOL,PAUWELSSTR 30 D-52057 AACHEN GERMANY RHEIN WESTFAL TH AACHEN,INST MED IMMUNOL D-52057 AACHEN GERMANY SMITHKLINE BEECHAM BIOL B-1330 RIXENSART BELGIUM
Titolo Testata:
Medical microbiology and immunology
fascicolo: 1, volume: 184, anno: 1995,
pagine: 17 - 22
SICI:
0300-8584(1995)184:1<17:MRTPOG>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR LEUKOCYTES; PERIPHERAL-BLOOD LEUKOCYTES; GROUP-A STREPTOCOCCI; M-PROTEIN; STAPHYLOCOCCUS-AUREUS; OPSONOPHAGOCYTOSIS; ATTACHMENT; INGESTION; KINETICS;
Keywords:
GROUP A STREPTOCOCCUS; GROUP G STREPTOCOCCUS; PHAGOCYTOSIS; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
N. Schnitzler et al., "MEASURING RESISTANCE TO PHAGOCYTOSIS OF GROUP-A AND GROUP-G STREPTOCOCCI - COMPARISON OF DIRECT BACTERICIDAL ASSAY AND FLOW-CYTOMETRY", Medical microbiology and immunology, 184(1), 1995, pp. 17-22

Abstract

M protein is thought to contribute to the ability of non-opsonized group A and group G streptococci (GAS and GGS, respectively) to resist phagocytosis by polymorphonuclear leukocytes. In previous studies, correlation between M protein expression and phagocytosis was determined by incubating these pathogens in human blood and comparing colony-forming bacterial counts prior to and after exposure to blood (direct bactericidal assay; DBA). Here, we report the application of flow cytometryto measure GAS and GGS resistance to phagocytosis. The results of theassays were in complete agreement with those from DBAs. Nevertheless,flow cytometry was regarded as superior to DBA because of its speed and potential uses for quantitative studies. In addition, the use of anti-CD 11b monoclonal antibody for granulocyte staining guaranteed a non-compromized granulocyte function. The optimized protocol for now cytometry presented here could be utilized to directly measure the involvement of individual protein types in bacterial resistance to phagocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 10:32:57