Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CHANGES IN LEVELS, SPECIES AND DNA FINGERPRINTS OF SOIL-MICROORGANISMS ASSOCIATED WITH COTTON EXPRESSING THE BACILLUS-THURINGIENSIS VAR KURSTAKI ENDOTOXIN
Autore:
DONEGAN KK; PALM CJ; FIELAND VJ; PORTEOUS LA; GANIO LM; SCHALLER DL; BUCAO LQ; SEIDLER RJ;
Indirizzi:
MANTECH ENVIRONM TECHNOL INC,200 SW 35TH ST CORVALLIS OR 97333 NATL RES COUNCIL CORVALLIS OR 97333 US EPA,ENVIRONM RES LAB CORVALLIS OR 97333
Titolo Testata:
Agriculture, ecosystems & environment. Applied soil ecology
fascicolo: 2, volume: 2, anno: 1995,
pagine: 111 - 124
SICI:
0929-1393(1995)2:2<111:CILSAD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIELD PERFORMANCE; PLANTS; INSECT; COMMUNITY; PROTEIN;
Keywords:
SOIL MICROBIOLOGY; GENETICALLY ENGINEERED PLANTS; RISK ASSESSMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
K.K. Donegan et al., "CHANGES IN LEVELS, SPECIES AND DNA FINGERPRINTS OF SOIL-MICROORGANISMS ASSOCIATED WITH COTTON EXPRESSING THE BACILLUS-THURINGIENSIS VAR KURSTAKI ENDOTOXIN", Agriculture, ecosystems & environment. Applied soil ecology, 2(2), 1995, pp. 111-124

Abstract

An important aspect of the risk assessment of pesticidal transgenic plants is the potential for detrimental effects on the soil ecosystem from residual plant material following harvesting and tillage. We evaluated this concern by placing leaves of three different lines of cottongenetically engineered to produce the Bacillus thuringiensis var. kurstaki (B.t.k.) endotoxin in soil and monitoring numbers and species ofindigenous soil bacteria and fungi. Four experiments, lasting 28 or 56 days, were performed using combinations of the following treatments:(1) soil only; (2) soil + purified B,t,k. toxin; (3) soil + parental cotton; (4) soil + purified B.t,k, toxin + parental cotton; (5) soil B,t.k, toxin-producing cotton. Two of the three transgenic cotton lines caused a transient increase in total bacterial and fungal population levels that was significantly higher on several sample days in the experiments than the levels in the other treatments. In contrast, neither the third transgenic cotton line nor the purified B.t,k. toxins hadany significant effects on the total numbers of bacteria and fungi. Transient changes in bacterial species composition, measured by biochemical tests of individual cultures, community substrate utilization andDNA fingerprinting, were also observed in treatments with the two transgenic plant lines. The plant line specificity of the response, and the lack of effects from the purified B,t,k, toxins, suggest that the observed effects of the two transgenic plant lines on soil microorganisms may not have resulted from the plants' production of B.t,k, toxin, We suggest that genetic manipulation or tissue culturing of the plantsmay have produced a change in plant characteristics, aside from B.t.k. toxin production, that can influence growth and species composition of soil microorganisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 09:37:28