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Titolo:
TRANSIENT CRE-SITE-BINDING AND KAPPA-B-SITE-BINDING IS CROSS-REGULATED BY CAMP-DEPENDENT PROTEIN-KINASE AND A PROTEIN PHOSPHATASE IN MOUSE SPLENOCYTES
Autore:
KOH WS; JEON YJ; HERRING AC; KAMINSKI NE;
Indirizzi:
MICHIGAN STATE UNIV,DEPT PHARMACOL & TOXICOL E LANSING MI 48824 MICHIGAN STATE UNIV,DEPT PHARMACOL & TOXICOL E LANSING MI 48824 KOREA ADV INST SCI & TECHNOL,DEPT BIOL SCI TAEJON 305701 SOUTH KOREA
Titolo Testata:
Life sciences
fascicolo: 6, volume: 60, anno: 1997,
pagine: 425 - 432
SICI:
0024-3205(1997)60:6<425:TCAKIC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC-AMP; TRANSCRIPTION FACTOR; INDUCIBLE TRANSCRIPTION; SOMATOSTATIN GENE; RESPONSE ELEMENT; ALPHA-SUBUNIT; ACTIVATION; IDENTIFICATION; CELLS; LYMPHOCYTES;
Keywords:
CAMP; LEUKOCYTES; CAMP RESPONSE ELEMENT BINDING PROTEIN (CREB); NUCLEAR FACTOR-KAPPA-B (NF-KAPPA-B); IMMUNE FUNCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
W.S. Koh et al., "TRANSIENT CRE-SITE-BINDING AND KAPPA-B-SITE-BINDING IS CROSS-REGULATED BY CAMP-DEPENDENT PROTEIN-KINASE AND A PROTEIN PHOSPHATASE IN MOUSE SPLENOCYTES", Life sciences, 60(6), 1997, pp. 425-432

Abstract

Cyclic AMP regulates a variety of cellular responses through activation of cAMP-dependent protein kinase (PKA). The catalytic subunit of PKA, in turn, activate cAMP responsive element (CRE) and nuclear factor-kappa B (NF-kappa B) binding proteins. In this study, we demonstrated that binding activity to both CRE and kappa B sites in nuclear extracts from spleen cells is modulated by PKA in a time-dependent manner. Electrophoretic mobility shift assays showed that binding by transcription factors to either the CRE or kappa B motif was rapidly up-regulatedby cAMP, with maximum binding detected at 30 min in response to forskolin stimulation of splenocytes. This was followed by a steady declinein CRE and kappa B thereafter reaching basal levels by 2 hr. This up-regulation in CRE and kappa B binding was closely associated with an enhancement of PKA activity which was maximum at 30 min following forskolin stimulation. However, unlike the binding of regulatory factors toCRE and kappa B motifs which was very transient, peak PKA activity was sustained for 2 hr. Interestingly, okadaic acid, a protein phosphatase inhibitor, prevented the decline in protein binding to CRE and kappa B motifs 2 hr following forskolin stimulation and actually produced a slight increase at 30 min. These data suggest that binding by transcription factors to CRE and kappa B sites are up-regulated concomitantly with PKA activation but subsequently down-regulated by a protein phosphatase.

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Documento generato il 20/09/20 alle ore 23:14:15