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Titolo:
MOLECULAR ANALYSIS OF THE MULTIPLE GOLF ALPHA-SUBUNIT MESSENGER-RNAS IN THE RAT-BRAIN
Autore:
HERVE D; ROGARD M; LEVISTRAUSS M;
Indirizzi:
COLL FRANCE,INSERM,U114,CHAIRE NEUROPHARMACOL,11 PL MARCELIN BERTHELOT F-75005 PARIS FRANCE UNIV PARIS 06,DEPT NEUROCHIM ANAT,CNRS,URA 1488 F-75005 PARIS FRANCE
Titolo Testata:
Molecular brain research
fascicolo: 1, volume: 32, anno: 1995,
pagine: 125 - 134
SICI:
0169-328X(1995)32:1<125:MAOTMG>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; SIGNAL-TRANSDUCTION; ADENYLYL CYCLASE; G-PROTEIN; EXPRESSION; SEQUENCES; SITE; TRANSCRIPT; RECEPTOR; STEP;
Keywords:
GTP-BINDING PROTEIN; SPLICING; POLYADENYLATION; STRIATUM; DOPAMINE; DOPAMINE D1 RECEPTOR; INTRON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
D. Herve et al., "MOLECULAR ANALYSIS OF THE MULTIPLE GOLF ALPHA-SUBUNIT MESSENGER-RNAS IN THE RAT-BRAIN", Molecular brain research, 32(1), 1995, pp. 125-134

Abstract

The alpha subunit of GTP-binding protein Golf (G alpha olf), identified in the olfactory epithelium, in which it is supposed to mediate odorant-generated adenylyl cyclase activations, is much more expressed inthe striatum than Gas, the classical stimulatory G protein a subunit (Herve et al., J Neurosci., 13 (1993) 2237-2248). Four species of G alpha olf messenger RNA (mRNA) were observed by Northern blot in the ratstriatum. Analysis of striatal G alpha olf cDNA clones demonstrated that despite extensive variations in their 5' and 3' untranslated regions, these four G alpha olf mRNAs encode the same G alpha olf polypeptide. One of the four mRNA species, which was selectively observed in the striatum, is generated by a novel promoter whose activity was barelydetectable in the olfactory epithelium. Surprisingly, this promoter generates an unexpectedly high proportion of transcripts in which the first intron is unspliced. The retention of intron was found to abolishthe translation of G alpha olf mRNA in the reticulocyte lysate system, suggesting that it could be involved in the down regulation of G alpha olf expression in the striatum. Furthermore, a new polyadenylation site with the non canonical sequence, AATACA, was found to be responsible of the two shortest forms of G alpha olf mRNA. In conclusion, we have shown that the G alpha olf proteins present in the striatum and the olfactory epithelium are identical and that multiple variations in the untranslated parts of the mRNAs could affect G alpha olf expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:38:13