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Titolo:
CONSTRUCTION OF ZN2-TOLERANT CYANOBACTERIA WITH A MODIFIED METALLOTHIONEIN DIVERGON - FURTHER ANALYSIS OF THE FUNCTION AND REGULATION OF SMT( CD2+)
Autore:
TURNER JS; ROBINSON NJ; GUPTA A;
Indirizzi:
UNIV NEWCASTLE,SCH MED,DEPT BIOCHEM & GENET NEWCASTLE TYNE NE2 4HH TYNE & WEAR ENGLAND
Titolo Testata:
Journal of industrial microbiology
fascicolo: 3-4, volume: 14, anno: 1995,
pagine: 259 - 264
SICI:
0169-4146(1995)14:3-4<259:COZCWA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROKARYOTIC METALLOTHIONEIN; SYNECHOCOCCUS STRAINS; ANACYSTIS-NIDULANS; LOCUS; IDENTIFICATION; CADMIUM; PROTEIN;
Keywords:
METAL-INDUCED-TRANSCRIPTION; METAL-RESISTANCE; SYNECHOCOCCUS SP; SMTA; SMTB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
J.S. Turner et al., "CONSTRUCTION OF ZN2-TOLERANT CYANOBACTERIA WITH A MODIFIED METALLOTHIONEIN DIVERGON - FURTHER ANALYSIS OF THE FUNCTION AND REGULATION OF SMT( CD2+)", Journal of industrial microbiology, 14(3-4), 1995, pp. 259-264

Abstract

This paper reports the (de novo) construction of mutants of Synechococcus PCC 7942 lacking the repressor (SmtB) of the metallothionein gene, smtA. These smA(+)/B- cells are more tolerant to elevated [Zn2+] and[Cd2+] than cells containing an intact metallothionein divergon (smt). Previously selected (by step-wise adaptation) Cd2+-tolerant mutants contain additional copies of smtA and possibly other undetected mutations. It is now confirmed that these cells also contain a deletion within 'all' copies of smtB and hence fail to revert to wild type following subculture in medium which has not been supplemented with Cd2+ or Zn2+. Northern analysis showed enhanced accumulation of smtA transcripts, even in the absence of added metal ions in these mutants. An increase in the accumulation of Zn2+ is reported in cells containing an intact metallothionein divergon compared to cells deficient in both smtA and smtB. This supports the assumption that SmtA binds Zn2+ within cyanobacterial cells. We also describe the use of the above mentioned mutants to identify additional factors involved in the regulation of transcription from the smtA operator-promoter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 04:40:41