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Titolo:
METALLOREGULATION OF THE CYANOBACTERIAL SMT LOCUS - IDENTIFICATION OFSMTB BINDING-SITES AND DIRECT INTERACTION WITH METALS
Autore:
ERBE JL; TAYLOR KB; HALL LM;
Indirizzi:
UNIV ALABAMA,DEPT BIOCHEM & MOLEC GENET BIRMINGHAM AL 35294 UNIV ALABAMA,DEPT BIOCHEM & MOLEC GENET BIRMINGHAM AL 35294
Titolo Testata:
Nucleic acids research
fascicolo: 13, volume: 23, anno: 1995,
pagine: 2472 - 2478
SICI:
0305-1048(1995)23:13<2472:MOTCSL>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROKARYOTIC METALLOTHIONEIN LOCUS; RESISTANCE OPERON; ESCHERICHIA-COLI; VIBRIO-CHOLERAE; GENE; EXPRESSION; PROTEIN; CADMIUM; LACKING; HLYU;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Erbe et al., "METALLOREGULATION OF THE CYANOBACTERIAL SMT LOCUS - IDENTIFICATION OFSMTB BINDING-SITES AND DIRECT INTERACTION WITH METALS", Nucleic acids research, 23(13), 1995, pp. 2472-2478

Abstract

The smtB gene of Synechococcus PCC 7942 encodes a trans-acting repressor of the metal-regulated smtA gene that encodes a class II metallothionein, Recombinant SmtB has been expressed in Escherichia coli and purified, Electrophoretic mobility shift assays using recombinant SmtB or a protein extract from Synechococcus PCC 6301 reveal the concentration-dependent formation of three specific complexes with the smt operator/promoter. SmtB is also capable of direct interaction with metals asevidenced by Zn-65 binding to the SmtB protein as well as the inhibition of repressor-DNA complex formation in the presence of various metal ions. Methylation interference analysis of such complexes identifiesfour protein contact points within the smt operator/promoter DNA, Thepoints of contact appear to represent two pairs of binding sites, onepair in each of two inverted repeats (nt 548-563, 589-602), The contact points within each pair lie on opposing DNA strands and are separated by 10 bp, placing the repressor binding sites on opposite sides of the DNA helix, Based on electrophoretic mobility shift assays, methylation interference and molecular size calculations we propose that recombinant SmtB binds to the smt operator/promoter in multimeric fashion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:15:55