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Titolo:
SUBSTANCE-P INDUCES ION SECRETION IN MOUSE SMALL-INTESTINE THROUGH EFFECTS ON ENTERIC NERVES AND MAST-CELLS
Autore:
WANG L; STANISZ AM; WERSHIL BK; GALLI SJ; PERDUE MH;
Indirizzi:
MCMASTER UNIV,FAC HLTH SCI,INTESTINAL DIS RES PROGRAMME,RM 3N5C,1200 MAIN ST W HAMILTON ON L8N 3Z5 CANADA MCMASTER UNIV,FAC HLTH SCI,INTESTINAL DIS RES PROGRAMME HAMILTON ON L8N 3Z5 CANADA BETH ISRAEL HOSP,COMBINED PROGRAM PEDIAT GASTROENTEROL & NUTR BOSTON MA 02115 BETH ISRAEL HOSP,DEPT PATHOL BOSTON MA 02115 HARVARD UNIV,SCH MED BOSTON MA 02115
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 1, volume: 32, anno: 1995,
pagine: 85 - 92
SICI:
0193-1857(1995)32:1<85:SIISIM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; GUINEA-PIG ILEUM; INFLAMMATORY MEDIATORS; NEUROKININ RECEPTORS; SELECTIVE AGONISTS; HISTAMINE-RELEASE; EPITHELIAL-CELLS; SENSORY NEURONS; JEJUNAL MUCOSA; DEFICIENT W/WV;
Keywords:
INTESTINAL EPITHELIUM; ION SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
L. Wang et al., "SUBSTANCE-P INDUCES ION SECRETION IN MOUSE SMALL-INTESTINE THROUGH EFFECTS ON ENTERIC NERVES AND MAST-CELLS", American journal of physiology: Gastrointestinal and liver physiology, 32(1), 1995, pp. 85-92

Abstract

We used genetically mast cell-deficient WBB6F(1) W/W-u (W/W-u) mice and congenic WBB6F(1) + / + normal (+ / +) mice to examine the role of mast cells in substance P-induced intestinal ion secretion. Isolated sheets prepared from segments of the midportion of the small intestine were studied in Ussing chambers. Substance P caused a dose-dependent increase in short-circuit current (I-sc) that was similar to 50% less in intestine from W/W-u than from + / + mice. Similar results were obtained for substance P-(4-11) (the COOH terminus) and substance P methylester [a selective neurokinin (NK)-1 agonist]. Histamine H-1 or H-2 antagonists reduced the I-sc responses to substance P in intestine from+ / + mice but had no effect in intestine from W/W-u mice. In addition, reconstitution of intestinal mast cells in W/W-u mice by intravenous injection of + / + bone marrow cells normalized the tissues' secretory responses to substance P or substance P methyl ester. However, in W/W-u and + / + mice, the selective NK1 antagonist CP-96345 virtually abolished intestinal responses to substance P, and the responses were also markedly inhibited by neural blockade with tetrodetoxin. In contrast, in tetrodotoxin-pretreated intestine, histamine antagonism caused a further reduction in the responses to substance P only in + / + mouse tissues. Taken together, our results suggest that the effects of substance P on intestinal I-sc responses are largely due to binding of the COOH terminus to NK1 receptors but that the neuropeptide acts via effects on enteric nerves and mast cells. The data thus support the concept that mast cells and enteric nerves participate in the regulation of substance P-induced intestinal ion secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:31:39