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Titolo:
ON THE BIOLOGICAL ROLE OF THE NUCLEAR POLYMERIZING NAD(-PROTEIN(ADP-RIBOSYL) TRANSFERASE (ADPRT) - ADPRT FROM DICTYOSTELIUM-DISCOIDEUM AND INACTIVATION OF THE ADPRT GENE IN THE MOUSE())
Autore:
AUER B; FLICK K; WANG ZQ; HAIDACHER D; JAGER S; BERGHAMMER H; KOFLER B; SCHWEIGER M; WAGNER EF;
Indirizzi:
INNSBRUCK UNIV,INST BIOCHEM,PETER MAYRSTR 1A A-6020 INNSBRUCK AUSTRIA RES INST MOLEC PATHOL A-1030 VIENNA AUSTRIA FREE UNIV BERLIN,INST BIOCHEM D-14195 BERLIN GERMANY
Titolo Testata:
Biochimie
fascicolo: 6, volume: 77, anno: 1995,
pagine: 444 - 449
SICI:
0300-9084(1995)77:6<444:OTBROT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING DOMAIN; HUMAN POLY(ADP-RIBOSE) POLYMERASE; MAMMALIAN-CELLS; DAMAGED DNA; RIBOSYLTRANSFERASE; REPAIR; EXPRESSION; INHIBITION;
Keywords:
DICTYOSTELIUM; GENE TARGETING; ADPRT NEGATIVE MOUSE; SKIN INTEGRITY; DNA REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
B. Auer et al., "ON THE BIOLOGICAL ROLE OF THE NUCLEAR POLYMERIZING NAD(-PROTEIN(ADP-RIBOSYL) TRANSFERASE (ADPRT) - ADPRT FROM DICTYOSTELIUM-DISCOIDEUM AND INACTIVATION OF THE ADPRT GENE IN THE MOUSE())", Biochimie, 77(6), 1995, pp. 444-449

Abstract

Two approaches have been used to elucidate the role of the nuclear polymerizing NAD(+):protein(ADP-ribosyl)transferase (ADPRT): i) comparison of the primary structure of Dictyostelium discoideum ADPRT derived from a 2 lib, partial cDNA sequence with the mammalian, fish, amphibian and insect counterparts revealed an overall homology of 25%. Whereasthe automodification domain was not conserved at all, the NAD(+) binding domain (aa 859-908) showed more than 70% identical amino acids in all species. Together with the similar enzymatic properties of the ADPRTs the genetic conservation underlined the notion that ADPRT plays a major role in various cellular processes; and ii) inactivation of the ADPRT gene in murine embryonic stem cells by homologous recombination led to mouse strains with a complete lack of nuclear poly(ADP-ribosyl)ation. These ADPRT mutant mice were viable and fertile indicating thatADPRT is dispensable in mouse development. Moreover, repair of UV andMNNG induced DNA damage was not affected in ADPRT/3T3 Like fibroblasts, as measured by reactivation of in vitro damaged reporter plasmids and unscheduled DNA synthesis. However, about 30% of the ADPRT mutant mice developed pathological skin aberrations on a mixed 129/Sv x C57B1/6 genetic background. These mice will be extremely useful to define the precise biological role of poly(ADP-ribosyl)ation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 02:20:27