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Titolo:
CRY-J-2, A MAJOR ALLERGEN OF JAPANESE CEDAR POLLEN, SHOWS POLYMETHYLGALACTURONASE ACTIVITY
Autore:
OHTSUKI T; TANIGUCHI Y; KOHNO K; FUKUDA S; USUI M; KURIMOTO M;
Indirizzi:
HAYASHIBARA BIOCHEM LABS INC,FUJISAKI INST,675-1 FUJISAKI OKAYAMA 702JAPAN OKINAKA MEM INST MED RES TOKYO 105 JAPAN
Titolo Testata:
Allergy
fascicolo: 6, volume: 50, anno: 1995,
pagine: 483 - 488
SICI:
0105-4538(1995)50:6<483:CAMAOJ>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYPTOMERIA-JAPONICA POLLEN; SUGI BASIC-PROTEIN; J-I; MONOCLONAL-ANTIBODIES; ANTIGENIC ANALYSES; POLYGALACTURONASE; EPITOPES; CLONING; LYASE;
Keywords:
ALLERGEN; CRY J 1; CRY J 2; JAPANESE CEDAR POLLEN; POLLINOSIS; POLYGALACTURONASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
T. Ohtsuki et al., "CRY-J-2, A MAJOR ALLERGEN OF JAPANESE CEDAR POLLEN, SHOWS POLYMETHYLGALACTURONASE ACTIVITY", Allergy, 50(6), 1995, pp. 483-488

Abstract

We examined Cry j 2, a major allergen of Japanese cedar (Cryptomeria japonica) pollen, for polygalacturonase enzyme activity, since a nucleotide sequence of cDNA of Cry j 2 showed a significant homology with that of tomato polygalacturonase. Polygalacturonase is well known to depolymerize preferentially polygalacturonic acid (PGA) by hydrolysis. However, Cry j 2 did not act on PGA, but was found to depolymerize pectin and methylesterified PGA in a dose-dependent manner. The substrate specificity of Cry j 2 was different from that of polygalacturonase derived from Aspergillus niger. The depolymerizing activity of Cry j 2 reached a maximum at 50%-60% of methylesterification of PGA. In contrast, polygalacturonase showed its maximum activity to PGA, and the activity decreased as the degree of methylesterification increased. Interestingly, the pectin-depolymerizing activity of Cry j 2 was due to a hydrolysis, but not a lyase, activity which splits the glycosidic bonds by beta-elimination, since no unsaturated uronides were found by measurement of absorbance at 235 nm in the reaction mixture. The enzyme activity was markedly inhibited by anti-Cry j 2 antibodies. These results indicate that Cry j 2 probably has polymethylgalacturonase enzyme activity, as postulated by von Neukom in 1963, although existence of this activity has not yet been proven.

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Documento generato il 27/11/20 alle ore 02:14:45