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Titolo:
BIOLOGICALLY-ACTIVE RECOMBINANT RICIN-B CHAIN PRODUCED IN ESCHERICHIA-COLI
Autore:
TONEVITSKII AG; TOPTYGIN AY; AGAPOV II; RAKHMANOVA VA; SHAMSHIEV AT; ALEKSEEV YO; PFUELLER V; FRANKEL A;
Indirizzi:
MOSCOW GENET & SELECT IND MICROORGANISMS INST MOSCOW 113545 RUSSIA RUSSIAN MINIST HLTH,INST IMMUNOL MOSCOW 115478 RUSSIA UNIV WITTEN HERDECKE,INST PHYTOCHEM W-5810 WITTEN GERMANY MED UNIV S CAROLINA,HOLLINGS ONCOL CTR CHARLESTON SC 29425
Titolo Testata:
Molecular biology
fascicolo: 2, volume: 29, anno: 1995,
parte:, 2
pagine: 235 - 241
SICI:
0026-8933(1995)29:2<235:BRRCPI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSE-BINDING ABILITY; A-CHAIN; EXPRESSION; TOXIN; IMMUNOTOXINS; CELLS; OLIGOSACCHARIDES; ANTIBODIES; COMMUNIS; CLONING;
Keywords:
RICIN; RECOMBINANT RICIN B SUBUNIT; ESCHERICHIA-COLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
A.G. Tonevitskii et al., "BIOLOGICALLY-ACTIVE RECOMBINANT RICIN-B CHAIN PRODUCED IN ESCHERICHIA-COLI", Molecular biology, 29(2), 1995, pp. 235-241

Abstract

A recombinant B subunit (rRTB) of ricin, a plant toxin, was obtained. The heterologous protein synthesized in Escherichia coli formed inclusion bodies. Denaturation and renaturation of the protein in the presence of glutathione and lactose yielded a soluble, biologically active and stable product. The rRTB had no toxic effect on HUT102 cells (ID50> 10(-6) M). Antigenic properties and the galactose-binding activity were the same for both natural and recombinant RTB; rRTB, however, proved to be more sensitive to trypsin. A chimeric toxin (rRTB-RTA) was reconstructed using rRTB and the natural ricin A subunit. The cytotoxicactivity of the rRTB-RTA heterodimer toward HUT102 cells was one-tenth that of the natural RTB-RTA heterodimer and ricin. These results suggest that carbohydrate chains bound to the RTB molecules are required to maintain the protein in a stable form and to prevent proteolysis ofthe protein in intracellular vesicles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:49:24