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Titolo:
IN-VIVO AND IN-VITRO FLOW-CYTOMETRY COMPARATIVE-ANALYSIS OF SOMATOSTATIN-POSITIVE CELLS IN THE PINEAL-GLAND OF THE NEONATAL RAT
Autore:
VIADER M; MATO E; TUGUES D; FORNAS O; PUIGDOMINGO M; WEBB SM;
Indirizzi:
AUTONOMOUS UNIV BARCELONA,HOSP SANT PAU,SERV ENDOCRINOL,EXPTL ENDOCRINOL LAB E-08025 BARCELONA SPAIN AUTONOMOUS UNIV BARCELONA,HOSP SANT PAU,SERV ENDOCRINOL,EXPTL ENDOCRINOL LAB E-08025 BARCELONA SPAIN HOSP DURAN & REYNALS,INST RECERCA ONCOL BARCELONA SPAIN
Titolo Testata:
Neuroendocrinology
fascicolo: 1, volume: 62, anno: 1995,
pagine: 87 - 92
SICI:
0028-3835(1995)62:1<87:IAIFCO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; N-ACETYLTRANSFERASE; IMMUNOREACTIVE SOMATOSTATIN; REGIONAL DISTRIBUTION; GENE-EXPRESSION; ONTOGENY; BRAIN; PEPTIDES; RECEPTORS; NEURONS;
Keywords:
SOMATOSTATIN; RAT; PINEAL; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M. Viader et al., "IN-VIVO AND IN-VITRO FLOW-CYTOMETRY COMPARATIVE-ANALYSIS OF SOMATOSTATIN-POSITIVE CELLS IN THE PINEAL-GLAND OF THE NEONATAL RAT", Neuroendocrinology, 62(1), 1995, pp. 87-92

Abstract

Flow cytometry was used for comparative in vivo and in vitro analysisof cell populations staining positively for somatostatin. Experimentswere carried out with pineals obtained from neonatal, 8- and 15-day-old rats. Pineal cells were obtained by dispersion with collagenase andthen processed in a flow cytometer or maintained in culture for 1 or 2 weeks. Identification of somatostatin-immunopositive cell populations was performed using a polyclonal somatostatin antibody and confirmedby indirect immunostaining of cytospun smears with the avidin-biotin-peroxidase method. In vivo, the percentage of somatostatin-positive cells was 60.6+/-4% in neonatal pineals and declined to 22.2+/-11% in 15-day-old animals (p < 0.04). The density of peptide immunostaining decreased in 8-day-old animals but recovered to the neonate levels in 15 day-old animals; homogeneity in the immunopositive population increased with age. Maintenance in culture for 1 week resulted in an increase in positive somatostatin staining in animals of 8 and 15 days with no changes in neonates; however, after 2 weeks of culture, the percent ofimmunopositive cells decreased from 53.3+/-6 to 12.2+/-4% in the older animals and remained unchanged in neonates. We conclude that somatostatin is found in pinealocytes and shows a declining pattern during the perinatal period; this probably implies that the peptide plays a paracrine role important for cell differentiation in these young animals,since maximal cellularity and a high mitotic index occur within the first 3 days of life, and pineal cell differentiation is completed before the end of the third week of extrauterine life.

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Documento generato il 01/12/20 alle ore 16:18:37