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Titolo:
A SOLUBLE FORM OF WNT-1 PROTEIN WITH MITOGENIC ACTIVITY ON MAMMARY EPITHELIAL-CELLS
Autore:
BRADLEY RS; BROWN AMC;
Indirizzi:
CORNELL UNIV,COLL MED,DEPT CELL BIOL & ANAT NEW YORK NY 10021 CORNELL UNIV,COLL MED,DEPT CELL BIOL & ANAT NEW YORK NY 10021 CORNELL UNIV,COLL MED,STRANG CORNELL CANC RES LAB NEW YORK NY 10021
Titolo Testata:
Molecular and cellular biology
fascicolo: 8, volume: 15, anno: 1995,
pagine: 4616 - 4622
SICI:
0270-7306(1995)15:8<4616:ASFOWP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ONCOGENE INT-1; WINGLESS PROTEIN; XENOPUS-LAEVIS; GENE FAMILY; EXPRESSION; DROSOPHILA; LINE; TRANSFORMATION; PROTOONCOGENE; ABNORMALITIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
R.S. Bradley e A.M.C. Brown, "A SOLUBLE FORM OF WNT-1 PROTEIN WITH MITOGENIC ACTIVITY ON MAMMARY EPITHELIAL-CELLS", Molecular and cellular biology, 15(8), 1995, pp. 4616-4622

Abstract

The proto-oncogene Wnt-1 plays an essential role in fetal brain development and causes hyperplasia and tumorigenesis when activated ectopically in the mouse mammary gland. When expressed in certain mammary epithelial cell lines, the gene causes morphological transformation and excess cell proliferation at confluence. Like other members of the mammalian Wnt family, Wnt-1 encodes secretory glycoproteins which have been detected in association with the extracellular matrix or cell surface but which have not previously been found in a soluble or biologically active form. We show here that conditioned medium harvested from a mammary cell line expressing Wnt-1 contains soluble Wnt-1 protein and induces mitogenesis and transformation of mammary target cells. By immunodepletion of medium containing epitope-tagged Wnt-1, we show that atleast 60% of this activity is specifically dependent on Wnt-1 protein. These results provide the first demonstration that a mammalian Wnt protein can act as a diffusible extracellular signaling factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 22:01:33