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Titolo:
INHIBITION OF IL-10 EXPRESSION BY IFN-GAMMA UP-REGULATES TRANSCRIPTION OF TNF-ALPHA IN HUMAN MONOCYTES
Autore:
DONNELLY RP; FREEMAN SL; HAYES MP;
Indirizzi:
US FDA,CTR BIOL EVALUAT & RES,DIV CYTOKINE BIOL,HFM-505,1401 ROCKVILLE PIKE ROCKVILLE MD 20852
Titolo Testata:
The Journal of immunology
fascicolo: 3, volume: 155, anno: 1995,
pagine: 1420 - 1427
SICI:
0022-1767(1995)155:3<1420:IOIEBI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; CYTOKINE PRODUCTION; INTERFERON-GAMMA; INTERLEUKIN-10 IL-10; RECEPTOR ANTAGONIST; FACTOR CACHECTIN; GENE-EXPRESSION; RELEASE; CELL; LIPOPOLYSACCHARIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
R.P. Donnelly et al., "INHIBITION OF IL-10 EXPRESSION BY IFN-GAMMA UP-REGULATES TRANSCRIPTION OF TNF-ALPHA IN HUMAN MONOCYTES", The Journal of immunology, 155(3), 1995, pp. 1420-1427

Abstract

Stimulation of human monocytes with LPS induces expression of multiple cytokines, including TNF-alpha, IL-1 beta, IL-6, and IL-10. IL-10 expression is delayed relative to that of TNF-alpha, IL-1 beta, and IL-6. Furthermore, IL-10 feedback inhibits expression of TNF-alpha, IL-1 beta, and IL-6, thus providing an efficient autocrine mechanism for controlling proinflammatory cytokine production in monocytes. The Th1-type lymphokine, IFN-gamma, markedly up-regulates TNF-alpha production inmonocytes. However, the precise mechanism by which IFN-gamma mediatesthis effect is unknown. We examined the effects of IFN-gamma on IL-10expression in LPS-stimulated monocytes, and the relationship between IL-10 and TNF-alpha production in these cells. LPS stimulation inducedrapid, ordered expression of multiple cytokines. Steady-state mRNA levels for TNF-alpha increased rapidly, reached maximal levels by 2 to 3h poststimulation, and then declined sharply. IL-1 beta and IL-6 mRNAlevels also increased markedly following stimulation with LPS, but decreased more slowly than did TNF-alpha. Down-regulation of mRNA for TNF-alpha, IL-1 beta, and IL-6 coincided with a delayed and more gradualincrease in IL-10 mRNA levels. Furthermore, neutralization of IL-10 with anti-IL-10 Abs prolonged TNF-alpha mRNA expression, and significantly increased net TNF-alpha production. IFN-gamma suppressed expression of IL-10 mRNA and protein in a dose-dependent manner. Moreover, inhibition of IL-10 production correlated with a marked increase in both the magnitude and duration of TNF-alpha expression. Thus, potentiation of TNF-alpha production by IFN-gamma in monocytes is coupled to inhibition of endogenous IL-10 expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 11:20:45