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Titolo:
SOMATIC AND GERMINAL ACTIVITIES OF MAIZE ACTIVATOR (AC) TRANSPOSASE MUTANTS IN TRANSGENIC TOBACCO
Autore:
KUNZE R; KUHN S; JONES JDG; SCOFIELD SR;
Indirizzi:
UNIV MUNICH,INST GENET & MIKROBIOL,MARIA WARD STR 1A D-80638 MUNICH GERMANY UNIV COLOGNE,INST GENET D-50931 COLOGNE GERMANY JOHN INNES INST,SAINSBURY LAB NORWICH NR4 7UH NORFOLK ENGLAND
Titolo Testata:
Plant journal
fascicolo: 1, volume: 8, anno: 1995,
pagine: 45 - 54
SICI:
0960-7412(1995)8:1<45:SAGAOM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZEA-MAYS-L; ELEMENT ACTIVATOR; DISSOCIATION EXCISION; ARABIDOPSIS-THALIANA; PUTATIVE TRANSPOSASE; MESSENGER-RNA; GENE; PLANTS; EXPRESSION; FREQUENCY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
R. Kunze et al., "SOMATIC AND GERMINAL ACTIVITIES OF MAIZE ACTIVATOR (AC) TRANSPOSASE MUTANTS IN TRANSGENIC TOBACCO", Plant journal, 8(1), 1995, pp. 45-54

Abstract

The properties have been investigated of two deletion derivatives of the transposase protein (TPase) of maize transposable element Ac in transgenic tobacco. The wildtype and mutant TPases were expressed as fusions to the cauliflower mosaic virus 35S promoter. A deletion of 102 amino acids from the N-terminus, TPase(103-807), induces Ds excisions from a SPT::Ds reporter locus with a higher frequency than the wild-type TPase. The increased transpositional activity of TPase(103-807) is adominant trait, as seedlings coexpressing truncated and wild-type TPase show the characteristic TPase(103-807) variegation phenotype. A transpositionally inactive TPase deletion derivative lacking 188 amino acids from the N-terminus inhibits the transpositional activity of the wild-type TPase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:31:02