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Titolo:
INFERTILITY IN A TRANSGENIC RAT DUE TO IMPAIRMENT OF CYTOPLASMIC ELIMINATION AND SPERM RELEASE FROM THE SERTOLI CELLS (VOL 53, PG 214, 1995)
Autore:
SHARPE RM; MAGUIRE SM; SAUNDERS PTK; MILLAR MR; RUSSELL LD; GANTEN D; BACHMANN S; MULLINS L; MULLINS JJ;
Titolo Testata:
Biology of reproduction
fascicolo: 2, volume: 53, anno: 1995,
pagine: 475 -
SICI:
0006-3363(1995)53:2<475:IIATRD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; GERM-CELLS; PLASMINOGEN-ACTIVATOR; SEMINIFEROUS TUBULES; CYCLIC PROTEIN-2; UROKINASE-TYPE; MESSENGER-RNA; ADULT-RATS; EXPRESSION; TESTIS;
Tipo documento:
Correction, Addition
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Sharpe et al., "INFERTILITY IN A TRANSGENIC RAT DUE TO IMPAIRMENT OF CYTOPLASMIC ELIMINATION AND SPERM RELEASE FROM THE SERTOLI CELLS (VOL 53, PG 214, 1995)", Biology of reproduction, 53(2), 1995, pp. 475

Abstract

In line TGR(mRen2)26 transgenic rats (TGR26) bearing a randomly inserted additional renin transgene, the males, but not the females, were found to be infertile. Tissue was obtained from TGR26 males and littermate controls after perfusion fixation, and the morphology of the testes and epididymides was examined. Testis size was normal as was gross morphology, but careful examination revealed that the release of many spermatozoa at stage IX of the spermatogenic cycle was impaired, In addition, the process of cytoplasmic elimination was abnormal, as cytoplasmic fragments of elongate spermatids were present in the epididymis, in TGR26 males, seminiferous tubule lumen size was significantly larger (p < 0.001) than in littermate controls, a difference that was most marked at stages IX-XIV-an effect that could be related to the retention of spermatozoa. In situ hybridization confirmed that expression of renin mRNA could be detected in testes of TGR26 rats but not in normalcontrols or in a fertile line (TGR27) of rats bearing the same transgene, Immunocytochemistry and in situ and Northern hybridization were used to elucidate the pattern of expression of genes that previous studies have implicated in the process of sperm maturation and/or release,Of the gene products examined (sulphated glycoproteins 1 and 2 [SGP-1, SGP-2], transition proteins 1 and 2 [TP-1, TP-2], urokinase, and cyclic protein 2 [CP-2]), none showed any major change in the pattern of expression compared with that in controls. We postulate that TGR26 transgenic male rats may be infertile because the expression of a gene (or genes) involved in the process of cytoplasmic elimination and/or sperm release has been disrupted by the presence of the transgene close to or within the gene(s). Future planned studies will involve determination of the insertion site(s) and ultrastructural analysis of the final phases of spermiogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:22:22