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Titolo:
OSTEOBLASTIC CELLS FROM RAT LONG-BONE .2. ADHESION TO SUBSTRATA AND INTEGRIN EXPRESSION IN PRIMARY AND PROPAGATED CULTURES
Autore:
CASTOLDI M; PISTONE M; CARUSO C; PUDDU A; FILANTI C; PICCINI D; TACCHETTI C; MANDUCA P;
Indirizzi:
UNIV GENOA,IST FISIOL I-16126 GENOA ITALY UNIV GENOA,IST ANAT I-16126 GENOA ITALY
Titolo Testata:
Cell biology international
fascicolo: 1, volume: 21, anno: 1997,
pagine: 7 - 16
SICI:
1065-6995(1997)21:1<7:OCFRL.>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
OSTEO-SARCOMA CELLS; ALKALINE-PHOSPHATASE ACTIVITY; HUMAN ENDOTHELIAL-CELLS; GLY-ASP PEPTIDE; EXTRACELLULAR-MATRIX; SIGNAL TRANSDUCTION; LAMININ RECEPTORS; SEQUENCE-ANALYSIS; FIBRONECTIN; DIFFERENTIATION;
Keywords:
OSTEOBLASTS; INTEGRINS; ADHESION; IN VITRO PROPAGATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M. Castoldi et al., "OSTEOBLASTIC CELLS FROM RAT LONG-BONE .2. ADHESION TO SUBSTRATA AND INTEGRIN EXPRESSION IN PRIMARY AND PROPAGATED CULTURES", Cell biology international, 21(1), 1997, pp. 7-16

Abstract

Propagation in vitro of rat tibial osteoblasts (ROB) is accompanied by increased expression of the early osteogenic marker alkaline phosphatase (AP) and maturation of the osteogenic phenotype. In order to establish the pattern of the integrin expressed in ROB during progression to the mature osteoblastic phenotype, we have used biosynthetic, immunoblotting and immunohistochemical assays. We immunoprecipitated from osteoblasts, expanded for 1.5- and 7.5-doubling, alpha 5 beta 1, alpha v beta 3, alpha 3 beta 1, alpha 6 beta 1 and alpha 1 beta 1 integrin heterodimers; furthermore beta 5, alpha 2 and alpha 4 chains were detected by immunoblots and indirect immunofluorescence. alpha v, alpha 1, alpha 6 subunits in most cells, and beta 3 and beta 1 subunits in a minority, were found to be associated with adhesion plaques in osteoblasts of 1.5-, 4.5- and 7.5-doubling grown in the presence of FCS, while all other subunits stained diffusely all the cells. Adhesion to fibronectin (FN), laminin (LN), collagen type I (COL I) and III (COL III) byROB at different doubling (1.5-11) was dependent on substratum concentration, and after 2.5 h at 55 nM 60% of the cells adhered to all substrata. Arg-Gly-Asp-Ser (RGDS) containing peptides inhibited adhesion of cells differentially, according to substratum; no dependence on extent of progation in vitro was observed. In conclusion, ROB cultured in vitro for 1.5- to 11-doubling had an unchanged pattern of expression of integrin subunits, heterodimer association and cellular distribution. Adhesion specificity and affinity were also unchanged. These resultssuggest that the phenotypic maturation, detected as an increase in APexpression, is not accompanied by major changes in the potential for cell-matrix interactions, and does not correspond to changes in the type of integrin subunits expressed by osteoblasts. (C) 1997 Academic Press Limited.

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Documento generato il 12/07/20 alle ore 12:46:56