Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ACTIVE CYCLIN B-CDC2 KINASE DOES NOT INHIBIT DNA-REPLICATION AND CANNOT DRIVE PREMATURELY FERTILIZED SEA-URCHIN EGGS INTO MITOSIS
Autore:
GENEVIEREGARRIGUES AM; BARAKAT A; DOREE M; MOREAU JL; PICARD A;
Indirizzi:
LAB ARAGO F-66650 BANYULS SUR MER FRANCE CNRS,CRBM F-34033 MONTPELLIER FRANCE
Titolo Testata:
Journal of Cell Science
, volume: 108, anno: 1995,
parte:, 7
pagine: 2693 - 2703
SICI:
0021-9533(1995)108:<2693:ACBKDN>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
EARLY STARFISH EMBRYO; CELL-CYCLE; FISSION YEAST; TYROSINE PHOSPHORYLATION; PROTEIN-KINASE; M-PHASE; XENOPUS-LAEVIS; CDC25 PROTEIN; S-PHASE; DIVISION CYCLE;
Keywords:
CELL CYCLE; CDC2; SEA URCHIN EGG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Genevieregarrigues et al., "ACTIVE CYCLIN B-CDC2 KINASE DOES NOT INHIBIT DNA-REPLICATION AND CANNOT DRIVE PREMATURELY FERTILIZED SEA-URCHIN EGGS INTO MITOSIS", Journal of Cell Science, 108, 1995, pp. 2693-2703

Abstract

Feedback mechanisms preventing M phase occurrence before S phase completion are assumed to depend on inhibition of cyclin B-cdc2 kinase activation by unreplicated DNA, In sea urchin, fertilization stimulates protein synthesis and releases eggs from G(1) arrest. We found that in the one-cell sea urchin embryo cyclin B-cdc2 kinase undergoes partial activation before S phase, reaching in S phase a level that is sufficient for G(2)-M phase transition. S phase entry is not inhibited by this level of cyclin B-dependent kinase activity, Inhibition of DNA replication by aphidicolin suppresses nuclear envelope breakdown, yet it does not prevent the microtubule array from being converted from its interphasic to its mitotic state, Moreover, mitotic cytoplasmic events occur at the same time in control and aphidicolin-treated embryos, Thus unreplicated DNA only prevents mitotic nuclear, not cytoplasmic, events from occurring prematurely, These results together show that the inhibition of cyclin B-cdc2 kinase activation is probably not the only mechanism that prevents mitotic nuclear events from occurring as long asDNA replication has not been completed. In contrast, cytoplasmic mitotic events seem to be controlled by a timing mechanism independent of DNA replication, set up at fertilization, that prevents premature opening of a window for mitotic events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:28:03