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Titolo:
INFECTION OF A HUMAN RESPIRATORY EPITHELIAL-CELL LINE WITH RHINOVIRUS- INDUCTION OF CYTOKINE RELEASE AND MODULATION OF SUSCEPTIBILITY TO INFECTION BY CYTOKINE EXPOSURE
Autore:
SUBAUSTE MC; JACOBY DB; RICHARDS SM; PROUD D;
Indirizzi:
JOHNS HOPKINS UNIV,CTR ASTHMA & ALLERGY,DEPT MED,DIV ALLERGY & CLIN IMMUNOL,OFF UNIT 2 BALTIMORE MD 21224 JOHNS HOPKINS UNIV,CTR ASTHMA & ALLERGY,DEPT MED,DIV ALLERGY & CLIN IMMUNOL,OFF UNIT 2 BALTIMORE MD 21224 JOHNS HOPKINS UNIV,CTR ASTHMA & ALLERGY,DEPT MED,DIV PULM & CRIT CAREMED BALTIMORE MD 21224
Titolo Testata:
The Journal of clinical investigation
fascicolo: 1, volume: 96, anno: 1995,
pagine: 549 - 557
SICI:
0021-9738(1995)96:1<549:IOAHRE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; SURFACE-RECEPTOR; COLDS; VIRUS; EXPRESSION; INTERFERON; NEUTROPHIL; ICAM-1; INTERLEUKIN-8; PRECIPITANTS;
Keywords:
COMMON COLD; ASTHMA; AIRWAY INFLAMMATION; ICAM-1; NEUTROPHILS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Subauste et al., "INFECTION OF A HUMAN RESPIRATORY EPITHELIAL-CELL LINE WITH RHINOVIRUS- INDUCTION OF CYTOKINE RELEASE AND MODULATION OF SUSCEPTIBILITY TO INFECTION BY CYTOKINE EXPOSURE", The Journal of clinical investigation, 96(1), 1995, pp. 549-557

Abstract

Rhinovirus infections cause over one third of all colds and are a contributing factor to exacerbations of asthma. To gain insights into theearly biochemical events that occur in infected epithelial cells, we develop, for the first time, a model in which a pure respiratory epithelial cell population can be routinely infected by rhinovirus. Viral infection was confirmed by demonstrating that viral titers of supernatants and lysates from infected cell increased with time and by PCR, Infection by rhinovirus 14 was inhibited by homotypic antiserum and by antibodies to intercellular adhesion molecule-1 (ICAM-1), the receptor for this virus. Susceptibility of epithelial cells to infection by rhinovirus 14 (but not rhinovirus 2, an ICAM-1 independent strain) can be increased by preexposure of cells to TNF alpha, whereas IFN gamma reduces susceptibility to infection by both rhinovirus strains. Rhinovirusinfection per se does not markedly alter ICAM-1 expression on epithelial cells. Finally, we demonstrate that rhinovirus infection induced increased production of IL-8, IL-6, and GM-CSF from epithelial cells. Production of IL-8 correlated with viral replication during the first 24 h after infection. This model should provide useful insights into the pathogenesis of rhinovirus infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 11:12:59