Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MECHANISM OF PROTECTION BY DIETHYLDITHIOCARBAMATE AGAINST CISPLATIN OTOTOXICITY - ANTIOXIDANT SYSTEM
Autore:
RYBAK LP; RAVI R; SOMANI SM;
Indirizzi:
SO ILLINOIS UNIV,SCH MED,DEPT PHARMACOL SPRINGFIELD IL 62794 SO ILLINOIS UNIV,SCH MED,DEPT PHARMACOL SPRINGFIELD IL 62794 SO ILLINOIS UNIV,SCH MED,DEPT SURG SPRINGFIELD IL 62794
Titolo Testata:
Fundamental and applied toxicology
fascicolo: 2, volume: 26, anno: 1995,
pagine: 293 - 300
SICI:
0272-0590(1995)26:2<293:MOPBDA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DOSE CISPLATIN; SULFUR-CONTAINING NUCLEOPHILES; INDUCED LIPID-PEROXIDATION; RAT-KIDNEY CORTEX; GLUTATHIONE-PEROXIDASE; THIOSULFATE PROTECTION; REDUCED GLUTATHIONE; SODIUM THIOSULFATE; CORTICAL SLICES; RESCUE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
L.P. Rybak et al., "MECHANISM OF PROTECTION BY DIETHYLDITHIOCARBAMATE AGAINST CISPLATIN OTOTOXICITY - ANTIOXIDANT SYSTEM", Fundamental and applied toxicology, 26(2), 1995, pp. 293-300

Abstract

This investigation was undertaken to explain the possible mechanism(s) of protection by diethyldithiocarbamate (DDTC) against cisplatin ototoxicity. Male Wistar rats (250-275 g) underwent pretreatment auditorybrain stem-evoked responses (ABRs). The different groups of rats wereinjected as follows: (1) cisplatin (16 mg/kg ip), (2) cisplatin plus DDTC (16 mg/kg ip + 600 mg/kg, sc), and (3) control rats. Post-treatment ABRs were performed after 3 days and the rats were euthanized and cochleae were harvested, The cochleae were analyzed for glutathione (GSH) and oxidized glutathione, by HPLC, and for the activities of the antioxidant enzymes, and malondialdehyde levels, by spectrophotometry. The cisplatin-injected rats showed a threshold elevation of 36 +/- 3.05dB above the pretreatment thresholds using click stimulus. Rats treated with cisplatin and then DDTC did not show a significant elevation of hearing threshold, DDTC-mediated protection was associated with higher levels of GSH (0.81 +/- 0.11 nmol/mg tissue), compared to 0.45 +/- 0.02 nmol/mg tissue following administration of cisplatin alone. Administration of cisplatin + DDTC restored the cochlear GSH-Px activity tocontrol level. Cisplatin-treated rats were found to have decreased GSH-Px activity (75% of control). Cochlear SOD and CAT activities and MDA levels showed a decreasing trend in the animals injected with cisplatin + DDTC, compared to cisplatin-alone-treated rats. These data suggest that the protection conferred by DDTC against cisplatin ototoxicityis associated with sparing of the cochlear GSH/GSH-Px. (C) 1995 Society of Toxicology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:42:49