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Titolo:
BENEFICIAL INFLUENCE OF VERO CELLS ON IN-VITRO MATURATION AND FERTILIZATION OF BOVINE OOCYTES
Autore:
GROCHOLOVA R; PETR J; MAREK J; TEPLA O;
Indirizzi:
INST CARE MOTHER & CHILD,PODOLSKE NAB 157 CR-14700 PRAGUE 4 CZECH REPUBLIC RES INST ANIM PROD PRAGUE 10 CZECH REPUBLIC
Titolo Testata:
Theriogenology
fascicolo: 2, volume: 44, anno: 1995,
pagine: 199 - 207
SICI:
0093-691X(1995)44:2<199:BIOVCO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO MATURATION; GRANULOSA-CELLS; EMBRYOS; COCULTURE; MONOLAYERS; IMPROVEMENT; BLASTOCYSTS; RESUMPTION; CLEAVAGE; MEIOSIS;
Keywords:
BOVINE; OOCYTE MATURATION; FERTILIZATION; COCULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R. Grocholova et al., "BENEFICIAL INFLUENCE OF VERO CELLS ON IN-VITRO MATURATION AND FERTILIZATION OF BOVINE OOCYTES", Theriogenology, 44(2), 1995, pp. 199-207

Abstract

The aim of this study was to examine the effects of Vero cells and other somatic cells on in vitro maturation of bovine oocytes. Both denuded oocytes and oocytes with intact cumuli (COCs) were cultured on monolayer of Vero cells, cumulus cells and granulosa cells. The effect of gonadotropins was investigated after the addition of gonadotropins to the culture medium. The evaluation using analysis of variance revealedthat removal of cumulus cells generally reduced the percentage of oocytes completing their maturation in vitro and that this effect could not be overcome by the addition of gonadotropins to the culture medium. However, in individual experiments, when oocytes were co-cultured with different monolayers of somatic cells, Vero cells were able significantly support the maturation of denuded oocytes, and their beneficial effect was further enhanced by the addition of gonadotropins (76 vs 80.9 %). We did not observe a similar effect after the co-culture of oocytes with a monolayer of cumulus cells (65.3 and 53 %, respectively). Granulosa cell monolayer delayed maturation in the both COCs and denuded oocytes (10.5 and 16.5 %, respectively). In vitro fertilization wassuccessful in most of the experimental groups. However, when denuded oocytes were cultured without any somatic cell support, they did not decondense the penetrated sperm head after in vitro fertilization. Thisstudy demonstrates that 1) Vero cells beneficially affect the in vitro maturation of bovine oocytes; 2) cumulus cells in the form of monolayer lose their beneficial influence on in vitro maturation of bovine oocytes; and 3) granulosa cells and FSH and LH alone (without somatic cells) do not show positive effects on in vitro maturation of bovine oocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 08:47:45