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Titolo:
LOW-FLOW HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SOLVENT DELIVERY SYSTEM DESIGNED FOR TANDEM CAPILLARY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY
Autore:
DAVIS MT; STAHL DC; LEE TD;
Indirizzi:
CITY HOPE NATL MED CTR,BECKMAN RES INST,DIV IMMUNOL,1450 E DUARTE RD DUARTE CA 91010 CITY HOPE NATL MED CTR,BECKMAN RES INST,DIV IMMUNOL DUARTE CA 91010
Titolo Testata:
Journal of the American Society for Mass Spectrometry
fascicolo: 7, volume: 6, anno: 1995,
pagine: 571 - 577
SICI:
1044-0305(1995)6:7<571:LHLSDS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROSPRAY INTERFACE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Davis et al., "LOW-FLOW HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SOLVENT DELIVERY SYSTEM DESIGNED FOR TANDEM CAPILLARY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY", Journal of the American Society for Mass Spectrometry, 6(7), 1995, pp. 571-577

Abstract

A solvent delivery system is described that is designed to increase the efficiency of liquid chromatography-mass spectrometry (LC/MS) analyses. Gradients formed by using two low pressure syringe pumps are stored in a length of narrow bore tubing (gradient loop) mounted on a standard high pressure switching valve. The preformed gradient is pushed through the column by using a high pressure syringe pump. The system isfully automated and can be controlled with either a personal computeror the mass spectrometer data system. Advantages include gradient operation without the use of split flows, pressure programed flow controlfor rapid sample loading and recycling to initial conditions, and a flow rate range of 0.1-20 mu L/min, which is suitable for packed capillary columns 50-500 mu m in diameter. The system has been used extensively for rapid molecular weight determinations of intact protein samples, as well as LC/MS and liquid chromatography-tandem mass spectrometryanalyses of complex peptide mixtures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 03:54:51