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Titolo:
CHARACTERIZATION OF MATERNAL AND ZYGOTIC D-RAF PROTEINS - DOMINANT-NEGATIVE EFFECTS ON TORSO SIGNAL-TRANSDUCTION
Autore:
RADKE K; BAEK KH; AMBROSIO L;
Indirizzi:
IOWA STATE UNIV,DEPT ZOOL & GENET,SIGNAL TRANSDUCT TRAINING GRP AMES IA 50011 IOWA STATE UNIV,DEPT ZOOL & GENET,SIGNAL TRANSDUCT TRAINING GRP AMES IA 50011
Titolo Testata:
Genetics
fascicolo: 1, volume: 145, anno: 1997,
pagine: 163 - 171
SICI:
0016-6731(1997)145:1<163:COMAZD>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASE; DROSOPHILA EMBRYOGENESIS; TERMINAL REGIONS; 14-3-3-PROTEINS; ACTIVATION; PATHWAY; PHOSPHORYLATION; REQUIREMENT; DOWNSTREAM; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
K. Radke et al., "CHARACTERIZATION OF MATERNAL AND ZYGOTIC D-RAF PROTEINS - DOMINANT-NEGATIVE EFFECTS ON TORSO SIGNAL-TRANSDUCTION", Genetics, 145(1), 1997, pp. 163-171

Abstract

The maternal D-raf serine/threonine kinase acts downstream of Torso (Tor) for specification of cell fates at the embryonic termini. D-raf activity is also required in other signal transduction pathways and consistent with its pleiotropic role, we find accumulation of a 90-kD D-raf protein throughout embryonic development. We also characterize the accumulation of maternal D-raf proteins in 0-2-hr embryos derived fromfemales with germ cells lacking D-raf activity. Accumulation of a 90-kD or truncated mutant D-raf protein is observed for some of these embryos, while others lack the maternal D-raf protein. Then, to determinewhether rescue of the Tor pathway is influenced by pools of nonfunctional maternal D-raf, wild-type D-raf mRNA was injected into embryos that inherit maternal stores of inactive 90-kD or truncated D-raf protein. For embryos lacking the maternal D-raf protein, a high level of terminal rescue is obtained. In contrast, rescue is reduced or not observed for embryos that accumulate mutant maternal D-raf proteins. These findings suggest that mutant forms of D-raf may deplete the embryo of apositive activator and/or form inactive protein complexes that affectrescue of the Tor pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 03:08:50