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Titolo:
BIO-AFFINITY CHARACTERIZATION MASS-SPECTROMETRY
Autore:
BRUCE JE; ANDERSON GA; CHEN RD; CHENG XH; GALE DC; HOFSTADLER SA; SCHWARTZ BL; SMITH RD;
Indirizzi:
PACIFIC NW LABS,DEPT CHEM SCI,CHEM METHODS & SEPARAT GRP RICHLAND WA 99352 PACIFIC NW LABS,DEPT CHEM SCI,CHEM METHODS & SEPARAT GRP RICHLAND WA 99352
Titolo Testata:
Rapid communications in mass spectrometry
fascicolo: 8, volume: 9, anno: 1995,
pagine: 644 - 650
SICI:
0951-4198(1995)9:8<644:BCM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTAGONIST; PEPTIDES; RECEPTOR; LIBRARY; LIGANDS; SPECTROSCOPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
J.E. Bruce et al., "BIO-AFFINITY CHARACTERIZATION MASS-SPECTROMETRY", Rapid communications in mass spectrometry, 9(8), 1995, pp. 644-650

Abstract

A new approach, bio-affinity characterization mass spectrometry (BACMS), aimed at providing a more rapid, sensitive and potentially more flexible alternative to techniques presently employed for the characterization of noncovalent interactions in mixtures, such as would be encountered in combinatorial chemistry, is presented, BACMS avoids some of the difficulties and potential artifacts associated with affinity chromatography since the noncovalent associations occur in solution; thus,BACMS avoids the requirement of solid support media and the development of non-interfering linker species. This paper describes the conceptual basis for the methodology and its potential use in applications which include the screening of high affinity ligands in support of new drug development. BACMS exploits new Fourier-transform ion cyclotron resonance (FTICR) mass spectrometry technologies which, when coupled to electrospray ionization (ESI), allow the investigation of specific noncovalent complexes formed in solution. BACMS utilizes the well-known attributes of FTICR, such as the high resolution mass analysis and (MS)(n) (n greater than or equal to 2) capabilities; however, it is even more directly a result of recently developed techniques involving quadrupolar excitation, such as selected-ion accumulation. These tools are demonstrated and the results illustrate the extraordinary sensitivity achievable (solution concentrations of 1x10(-9) M without the use of separations prior to ESI). Thus, the new capabilities demonstrated here, in conjunction with ESI, will be useful for the investigation of very low relative concentration noncovalent association directly from solution, and promote a faster alternative for combinatorial mixture screening and analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:09:41