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Titolo:
THE NONSTRUCTURAL PROTEINS OF THE AUTONOMOUS PARVOVIRUS MINUTE VIRUS OF MICE INTERFERE WITH THE CELL-CYCLE, INDUCING ACCUMULATION IN G(2)
Autore:
DEBEECK AO; ANOUJA F; MOUSSET S; ROMMELAERE J; CAILLETFAUQUET P;
Indirizzi:
FREE UNIV BRUSSELS,DEPT BIOL MOLEC,67 RUE CHEVAUX B-1640 RHODE ST GENESE BELGIUM DEUTSCH KREBSFORSCHUNGSZENTRUM,DEPT APPL TUMOR VIROL,ABT 0610 D-69120HEIDELBERG GERMANY DEUTSCH KREBSFORSCHUNGSZENTRUM,INSERM,U375 D-69120 HEIDELBERG GERMANY
Titolo Testata:
Cell growth & differentiation
fascicolo: 7, volume: 6, anno: 1995,
pagine: 781 - 787
SICI:
1044-9523(1995)6:7<781:TNPOTA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENO-ASSOCIATED VIRUS; TRANSFORMED RAT-CELLS; TRANS-ACTIVATION; GENE-EXPRESSION; SIMIAN VIRUS-40; DNA-REPLICATION; POLYOMA-VIRUS; NS-1 PROTEIN; REP PROTEIN; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
A.O. Debeeck et al., "THE NONSTRUCTURAL PROTEINS OF THE AUTONOMOUS PARVOVIRUS MINUTE VIRUS OF MICE INTERFERE WITH THE CELL-CYCLE, INDUCING ACCUMULATION IN G(2)", Cell growth & differentiation, 6(7), 1995, pp. 781-787

Abstract

The nonstructural (NS) proteins of the autonomous parvovirus minute virus of mice (prototype strain) are involved in viral DNA replication and in the regulation of parvoviral and heterologous promoters. By constructing cell lines having integrated the NS coding sequence under the control of an inducible promoter, we were able to demonstrate that NS proteins are toxic, once expressed in the transformed cells. Cell killing appears after several days of NS expression, suggesting that NS toxicity involves cellular factors. In this paper, we show that NS proteins are cytotoxic and interfere with the cell cycle in proliferatingcells only. NS expression is innocuous in resting cells, whereas in growing cells, it induces the accumulation of G(2) cells. This cytostatic effect is enhanced upon neoplastic transformation, which sensitizesthe cells to NS killing. Moreover, as clones resistant to NS toxicityundergo no alteration of their cycle, this cytostatic effect of NS proteins could be an early step on the way to cell killing. These observations strongly suggest that NS toxicity involves cellular factors associated with the regulation of the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:25:14