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Titolo:
PURIFICATION AND CHARACTERIZATION OF THROMBOPOIETIN
Autore:
KATO T; OGAMI K; SHIMADA Y; IWAMATSU A; SOHMA Y; AKAHORI H; HORIE K; KOKUBO A; KUDO Y; MAEDA E; KOBAYASHI K; OHASHI H; OZAWA T; INOUE H; KAWAMURA K; MIYAZAKI H;
Indirizzi:
KIRIN BREWERY CO LTD,PHARMACEUT RES LAB,1-2-2 SOUJAMACHI MAEBASHI GUMMA 371 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
Journal of Biochemistry
fascicolo: 1, volume: 118, anno: 1995,
pagine: 229 - 236
SICI:
0021-924X(1995)118:1<229:PACOT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; RECEPTOR SUPERFAMILY; C-MPL; PROTEINS; PLASMA; ERYTHROPOIETIN; SERUM; CELLS; MICE; GENE;
Keywords:
C-MPL LIGAND; MEGAKARYOCYTE; PLASMA PROTEIN PURIFICATION; PLATELET; THROMBOPOIETIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
T. Kato et al., "PURIFICATION AND CHARACTERIZATION OF THROMBOPOIETIN", Journal of Biochemistry, 118(1), 1995, pp. 229-236

Abstract

A thrombopoietic factor, termed thrombopoietin (TPO), was highly purified directly from the plasma of sublethally irradiated 1,100 rats by measuring the production of megakaryocytes from a highly enriched population of rat megakaryocyte progenitor cells (CFU-MK), The rat plasma TPO is a glycoprotein and strongly hydrophobic, The total activity andpurification yields obtained were about 29% and 1.49X10(8), respectively. The amino acid sequences of the two peptide fragments prepared from the purified 19 kDa TPO were analyzed, and used for the cloning of rat and human TPO cDNAs, It was found that the 19 kDa TPO was truncated but comprised at least 163 amino acids, The sequence of human TPO cDNA revealed that the TPO was identical to the c-Mpl ligand, Both rat and human TPOs expressed in COS-1 cells exhibited significant activity toward the CFU-MK in vitro, and were active in stimulating platelet production in mice, These results indicate that a thrombopoietic factor originally found in the irradiated rat plasma is a ligand for the rat c-Mpl.

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Documento generato il 18/01/21 alle ore 14:15:06