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Titolo:
ACTIVATION OF ADENYLATE-CYCLASE BY DIVALENT-CATIONS AND POLYAMINES INSAPONIN-TREATED DICTYOSTELIUM-DISCOIDEUM CELLS
Autore:
OYAMA M; KUBOTA K;
Indirizzi:
HIMEJI INST TECHNOL,FAC SCI,DEPT LIFE SCI,SHOSHA 2167 HIMEJI HYOGO 67122 JAPAN
Titolo Testata:
Journal of Biochemistry
fascicolo: 1, volume: 118, anno: 1995,
pagine: 117 - 121
SICI:
0021-924X(1995)118:1<117:AOABDA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLULAR SLIME-MOLD; REAGENT-INDUCED ACTIVATION; CAMP SIGNALING RESPONSE; CYCLIC-GMP FORMATION; INOSITOL 1,4,5-TRISPHOSPHATE; GUANYLATE-CYCLASE; PLASMA-MEMBRANE; ADENOSINE; ACCUMULATION; RECEPTORS;
Keywords:
ADENYLATE CYCLASE; CA2+; DICTYOSTELIUM DISCOIDEUM; POLYAMINE; SAPONIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Oyama e K. Kubota, "ACTIVATION OF ADENYLATE-CYCLASE BY DIVALENT-CATIONS AND POLYAMINES INSAPONIN-TREATED DICTYOSTELIUM-DISCOIDEUM CELLS", Journal of Biochemistry, 118(1), 1995, pp. 117-121

Abstract

Binding of an intrinsic agonist (cyclic AMP) to specific receptors onthe cell surface induces transmembrane signals for the activation of adenylate cyclase in the cellular slime mold Dictyostelium discoideum. We found that stimulation by CaCl2, MgSO4 or polyamines having two tofour positive charges induced the activation of adenylate cyclase in cells treated with saponin, The activation was roughly identical to the stimulation of the intrinsic agonist both in amount and in time course, The intact cells (saponin-untreated cells) responded to neither divalent cations nor polyamines. While saponin is known to have a detergent-like effect and to make the plasma membrane permeable, low molecular weight dyes did not penetrate the plasma membrane under our conditions for the saponin-treatment, Caffeine is known to inhibit the cAMP-induced activation of adenylate cyclase by blocking signal transduction, but not by acting directly on the enzyme [Brenner, M. and Thoms, S.D. (1984) Dev. Biol. 101, 136-146], We found that caffeine inhibited the cation-induced activation, These results suggest that these divalentand polyvalent cations do not act directly on adenylate cyclase but that they mimic or induce the transmembrane activation signal for adenylate cyclase in the saponin-treated cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 05:13:56