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Titolo:
HOE-694 BLOCKS NA+ H+ EXCHANGE IN HUMAN B-LYMPHOBLASTS WITHOUT INFLUENCING PROLIFERATION/
Autore:
ROSSKOPF D; SCHOLZ W; LANG HJ; SCHOLKENS BA; SIFFERT W;
Indirizzi:
UNIV KLINIKUM ESSEN,INST PHARMAKOL,HUFELANDSTR 55 D-45122 ESSEN GERMANY HOECHST AG W-6230 FRANKFURT GERMANY
Titolo Testata:
Cellular physiology and biochemistry
fascicolo: 4, volume: 5, anno: 1995,
pagine: 269 - 275
SICI:
1015-8987(1995)5:4<269:HBNHEI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMILORIDE ANALOGS; PHORBOL ESTER; INHIBITION; CELLS; HYPERTENSION; FIBROBLASTS; ISOFORMS; CULTURE; HOE-694; SYSTEM;
Keywords:
NA+/H+ EXCHANGE; INHIBITOR; PH REGULATION; CELL PROLIFERATION; LYMPHOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
D. Rosskopf et al., "HOE-694 BLOCKS NA+ H+ EXCHANGE IN HUMAN B-LYMPHOBLASTS WITHOUT INFLUENCING PROLIFERATION/", Cellular physiology and biochemistry, 5(4), 1995, pp. 269-275

Abstract

The effect of HOE 694 (3-methylsulfonyl-4-piperidinobenzoyl guanidinehydrochloride), a novel inhibitor of Na+/H+ exchange, on pH(i) recovery from acidification and on proliferation of human B lymphoblasts wasinvestigated. HOE 694 inhibited the Na+/H+ exchanger in a concentration-dependent manner. Inhibition constants (K-i) were calculated at 89 /- 5 and 54 +/- 7 nmol/l (means +/- SD; n = 5) at 135 and 79 mmol/l external Na+, respectively. This indicates a competition of HOE 694 with external Na+ ions at a common binding site of the Na+/H+ exchanger. Subsequently, the effects of HOE 694 upon DNA synthesis and proliferation of lymphoblasts were examined. HOE 694 inhibited cell proliferation and DNA synthesis only at concentrations of > 10 mu mol/l. This suggests that in the presence of bicarbonate a functionally active Na+/H+ exchanger is not required for the proliferation of B lymphoblasts.

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Documento generato il 31/03/20 alle ore 18:28:07