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Titolo:
TRANSPLANTATION OF FETAL PORCINE PANCREAS TO DIABETIC OR NORMOGLYCEMIC NUDE-MICE - EVIDENCE OF A RAPID ENGRAFTMENT PROCESS DEMONSTRATED BY BLOOD FLOW AND HEAT SHOCK PROTEIN-70 MEASUREMENTS
Autore:
SANDBERG JO; MARGULIS B; JANSSON L; KARLSTEN R; KORSGREN O;
Indirizzi:
UNIV UPPSALA,DEPT MED CELL BIOL,POB 571 S-75123 UPPSALA SWEDEN UNIV UPPSALA,DEPT ANESTHESIOL S-75123 UPPSALA SWEDEN UNIV UPPSALA,DEPT CLIN IMMUNOL & TRANSFUS MED S-75123 UPPSALA SWEDEN RUSSIAN ACAD SCI,INST CYTOL ST PETERSBURG 194064 RUSSIA
Titolo Testata:
Transplantation
fascicolo: 12, volume: 59, anno: 1995,
pagine: 1665 - 1669
SICI:
0041-1337(1995)59:12<1665:TOFPPT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LASER DOPPLER MEASUREMENTS; ISLET TRANSPLANTATION; BIOLOGICAL ZERO; STRESS PROTEINS; CELL CLUSTERS; BETA-CELL; HYPERGLYCEMIA; RATS; PERFUSION; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
J.O. Sandberg et al., "TRANSPLANTATION OF FETAL PORCINE PANCREAS TO DIABETIC OR NORMOGLYCEMIC NUDE-MICE - EVIDENCE OF A RAPID ENGRAFTMENT PROCESS DEMONSTRATED BY BLOOD FLOW AND HEAT SHOCK PROTEIN-70 MEASUREMENTS", Transplantation, 59(12), 1995, pp. 1665-1669

Abstract

The aims of the present investigation were to study the blood flow rate in transplanted fetal porcine islet-like cell clusters (ICC) with laser-Doppler flownmetry and to evaluate the influence of hyperglycemiaon this process. In order to investigate the degree of stress in transplanted beta cells during engraftment, the amount of heat shock proteins (HSP) or stress proteins was measured, ICC were prepared from the fetal porcine pancreas by employing a tissue culture technique. A total volume of 6 mu l (-750) ICC was transplanted under the left kidney capsule of either normo- or hyperglycemic C57BL/6J nude mice, The bloodflow to the xenogeneic islet graft and the adjacent kidney parenchymawere measured with laser Doppler flowmetry (LDF), Within 2 hr after transplantation (day 0) or 3, 6, 9, 12 (both normo- and hyperglycemic recipients), 15, 40, or 85 (only normoglycemic recipients) days after implantation of ICC into the nude mice, the hsp70 contents of the grafts were analyzed by means of an immunoassay, Revascularization of the subcapsular ICC graft was initiated rapidly, and 3 days posttransplantation the nutritional blood supply constituted 70% of the adjacent kidney blood flow, This figure increased with time after implantation until the IGC graft had equal or even higher blood perfusion than the adjacent kidney parenchyma, Hyperglycemia in recipients did not affect therevascularization process, Hsp analysis from ICC in culture demonstrated a surprisingly high content of hsp70, However, 3-9 days after transplantation the hsp70 content was markedly reduced in both the normo- and hyperglycemic group and then remained low in both groups. Thus, the present study showed that ICC transplanted into normo- or hyperglycemic nude mice were rapidly revascularized, and that the production of hsp70 was lowered already 3 days after transplantation when compared with cultured ICCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 06:10:35