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Titolo:
RETENTION OF LONG-TERM REPOPULATING ABILITY OF XENOGENEIC TRANSPLANTED PURIFIED ADULT HUMAN BONE-MARROW HEMATOPOIETIC STEM-CELLS IN SHEEP
Autore:
ZANJANI ED; SROUR EF; HOFFMAN R;
Indirizzi:
UNIV NEVADA,DEPT VET AFFAIRS MED CTR,SCH MED,151,1000 LOCUST ST RENO NV 89520 INDIANA UNIV,SCH MED,HEMATOL ONCOL SECT INDIANAPOLIS IN 00000 SYSTEMIX INC PALO ALTO CA 00000
Titolo Testata:
The Journal of laboratory and clinical medicine
fascicolo: 1, volume: 126, anno: 1995,
pagine: 24 - 28
SICI:
0022-2143(1995)126:1<24:ROLRAO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-FORMING CELLS; PROGENITOR CELLS; GROWTH-FACTORS; INUTERO; ENGRAFTMENT; EXPRESSION; DIFFERENTIATION; PRECURSORS; CHIMERAS; CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
E.D. Zanjani et al., "RETENTION OF LONG-TERM REPOPULATING ABILITY OF XENOGENEIC TRANSPLANTED PURIFIED ADULT HUMAN BONE-MARROW HEMATOPOIETIC STEM-CELLS IN SHEEP", The Journal of laboratory and clinical medicine, 126(1), 1995, pp. 24-28

Abstract

We have previously reported on the successful engraftment and long-term multilineage expression (erythroid, myeloid, lymphoid) of human adult marrow CD34(+)HLA-DR(-) cells in sheep after transplantation in utero. The persistence in these animals of CD34(+)HLA-DR(-) cells, the phenotype of the original graft, and the presence of assayable high proliferative potential colony-forming cells (HPP-CFCs), a primitive progenitor cell that has self-renewal capacity and multilineage differentiative capacity, suggested that these chimeric animals continued to contain human pluripotent hematopoietic stem cells (HSCs). To test this hypothesis, CD45(+) cells from the bone marrow of such an animal were isolated by panning at 19 and 24 months after birth, were pooled, and were transplanted into 4 preimmune sheep fetuses (0.6 x 10(6) viable cells per fetus). Three of the sheep were born alive, Bone marrow from one of these secondary recipients (lamb 58) exhibited significant donor (human) cell presence at 3, 9, 12, and 15 months (last evaluation period) after transplant, The engraftment was sustained along with multilineage expression involving lymphoid, erythroid, and myeloid cells of human origin. These results indicated that human CD34(+)HLA-DR(-) cellsare capable of long-term marrow repopulation and that this cell population is enriched for human long-term repopulating HSCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 06:29:04